Hanh Lam Email & Phone Number

United States

Blacksburg, Virginia, United States

United States

Development of validated probes for the bacterial type III secretion system

United States

I have been mentoring more than 15 undergraduate students at UC Santa Cruz, working on the the Type III secretion system inhibitor project

1156 High Street

Discovery and development of novel antimicrobialsIn the last several decades, more and more multi-drug resistance bacteria have been detected, but very few new antibiotics have entered the clinic and very few research dollars have been spent on development of next-generation antimicrobial agents. Recent report of the World Health Organization shows that global emergence of antibiotic resistant bacteria is posing a serious threat to the world public health. The more antibiotic resistant strains emerge, the fewer our drug choices and more expensive the drugs are. Modern medical advances such as surgery and transplants may face threats of infection. Antimicrobial research is thus pressingly demanded. A number of bacterial pathogens require the type III secretion system (T3SS) to grow inside host organisms. Fortunately, T3SS is largely absent in beneficial and non-pathogenic bacteria. This makes the T3SS an ideal target for novel antimicrobial agents. I am developing a high throughput screen to identify a suite of small molecules that can inhibit T3SS for use as new therapeutic candidates.

Ithaca, Ny

I am a PhD candidate in Plant-Microbe Biology at Cornell University with concentrations in Computational Biology and Genomics

Ithaca, Ny, Usa

Virulence regulation in the plan pathogen Pseudomonas syringae pv.tomato DC3000The type III secretion system, required for suppression of plant defense and pathogenicity, is directly regulated by a sigma factor HrpL. HrpL regulon has long been a subject of several studies. RpoN is the sigma factor responsible for hrpL transcription. RpoN regulon is of great interest because RpoN is a global regulator for bacterial growth, survival and response to different environmental changes and presumably it coordinates the expression of other pathogenesis-related genes in parallel with those downstream of HrpL.Characterization of the HrpL regulon:• Globally mapping HrpL binding sites (using ChIP-Seq) and transcription of downstream genes (using RNA-Seq) across the DC3000.• Studying the roles of HrpL regulated genes in pathogenicity through mutational analysis.• Trapping promoters upstream of green fluorescence proteinreporter to search for functional promoters• Characterizing a novel effector protein.Characterization of the RpoN regulon:• Finding RpoN regulated genes using ChIP-Seq and RNA-Seq analysis.• Finding differentially expressed genes as pathogenicity related candidates

Supervised a genetics major, HoangChuong BuiNguyen, on characterization of HrpL and RpoN regulon: explaining methods and concepts, demonstrating techniques, observing the learner and discussing results.

Teaching Assistant in Applied Bioinformatics and Systems Biology (BTRY6700): holding office hours, holding weekly lab section, grading homework and final, designing exam questions.

Vietnam

I worked as a Research Assistant and lecturer of Molecular Biology techniques in the Research Institute of Biotechnology and Environment, Nong Lam University• Supervised senior Biotechnology majors to conduct undergraduate research theses. Designing experiments, demonstrating techniques, and discussing results• Teaching Molecular Biology Techniques. Discussing methods, preparing materials for and assisting with lab sections.

Vietnam

Conducting a diagnosis and survey on the prevalence of Tobacco Mosaic Virus, Cucumber Mosaic Virus and Tomato Spotted Wilt Virus using Enzyme linked Immuno - Sorbent Assay (ELISA) and Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Doctor Of Philosophy (Phd), Plant-Microbe Biology

Activities and Societies: • The American Association for the Advancement of Science (AAAS) • The American Society for Microbiology (ASM)We.

Bachelor'S Degree, Biotechnology, Top 10 Of Class (90 Students)

Activities and Societies: I was a captain of the Volleyball team of our Department and University. I was an active member of the Youth.