Harrison Rohrer Email & Phone Number

Medford, Ma, Us

Boston, Massachusetts, United States

Lexington, Massachusetts, United States

I validated novel mRNA vaccine antigen designs through in vitro potency assays (primarily flow cytometry and western blots) using mRNA I generated via cloning and IVT and transfected into adherent cell culture plates. I also produced recombinant proteins for many of these antigens in suspension cell culture flasks at up to 1L scale and purified them using size-exclusion chromatography for characterization with bio-layer interferometry and thermal shift assays to determine binding kinetics and… Show more I validated novel mRNA vaccine antigen designs through in vitro potency assays (primarily flow cytometry and western blots) using mRNA I generated via cloning and IVT and transfected into adherent cell culture plates. I also produced recombinant proteins for many of these antigens in suspension cell culture flasks at up to 1L scale and purified them using size-exclusion chromatography for characterization with bio-layer interferometry and thermal shift assays to determine binding kinetics and melting temperature.By collaborating frequently with colleagues working on other teams, I applied creative solutions from different projects to my own work and vice versa, whether that be for assay development and refinement or data analysis. I also became very comfortable providing training on instruments and techniques to other researchers, and frequently either generated mRNA and protein for other teams to use and analyze or ran assays for other teams using mRNA and protein they provided from their own work.My other main responsibilities included adherent and suspension cell culture maintenance for multiple mammalian cell lines, mRNA transfection using both in-house formulations and commercial transfection reagents, cloning workflows using modern PCR, Gibson, and Golden Gate assembly techniques, plasmid preparation for storage and sequencing, sequence analysis and validation, data processing and visualization, and meticulous maintenance of electronic lab notebooks. Show less

I taught AP Environmental Science, Advanced Algebra 1, and Algebra 2, and created my own lesson plans using resources from peers, online directories, and my own experiences as a student.I also coached cross country and alpine skiing, served as faculty advisor to the student senate, was a member of the faculty professional learning community on equity and inclusion in the school, and helped lead a trip through the National Parks of Utah and Arizona in Spring 2022.As part of the… Show more I taught AP Environmental Science, Advanced Algebra 1, and Algebra 2, and created my own lesson plans using resources from peers, online directories, and my own experiences as a student.I also coached cross country and alpine skiing, served as faculty advisor to the student senate, was a member of the faculty professional learning community on equity and inclusion in the school, and helped lead a trip through the National Parks of Utah and Arizona in Spring 2022.As part of the eighth grade advising team, I worked with seven of my eighth grade students throughout the school year on their growth as students, leaders, and members of the school community. Show less

Cambridge, Massachusetts, United States

Volunteer instructor / facilitator of week-long virtual programs designed to introduce medical scenarios to high school students in the Boston area and beyond- Helped teach, debrief, and act out mock cases like asthma attacks, gall stones, and bike accidents to create engaging lessons on human anatomy and disease

Boston, Massachusetts, United States

I helped explore epigenetic regulators of pancreatic cancer development in mouse, chicken egg, and patient-derived cell models, working independently and as an assistant to postdoctoral researchers under the guidance of Andrew Liss, PhD and Carlos Fernandez del Castillo, MD (summers only).During my time there, I developed proficiency with mammalian cell culture maintenance, DNA/RNA/protein extraction and quantification, western blotting, immunohistochemistry, cloning, oligonucleotide… Show more I helped explore epigenetic regulators of pancreatic cancer development in mouse, chicken egg, and patient-derived cell models, working independently and as an assistant to postdoctoral researchers under the guidance of Andrew Liss, PhD and Carlos Fernandez del Castillo, MD (summers only).During my time there, I developed proficiency with mammalian cell culture maintenance, DNA/RNA/protein extraction and quantification, western blotting, immunohistochemistry, cloning, oligonucleotide ligation assays, SDM transformation, nucleofection, and qPCR techniques. I also gained experience with mouse injections and tumor removal and re-organized the lab's chemical inventory. Show less

Crested Butte, Colorado, United States

Served as assistant guide to hiking, rafting, and climbing trips based out of the Taylor River Lodge- Managed on-site activities, running and maintaining the archery, axe-throwing, climbing, etc. stations- Created manual of intern duties and protocols, and consolidated property inventory documents- Assisted hospitality services with landscaping, maintenance, car detailing, room re-stocking, etc. when not engaged in guiding and other guest-facing duties