Harthik Reddy Email & Phone Number

Boston, Massachusetts, United States

Boston, Massachusetts, United States

• Assisted the supervisor in design and execution of experiments for optimizing AAV vector production.• Monitored and maintained mammalian cell culture processes in up to 12 x 2L Finesse bioreactors that include setup, inoculation, sampling, conducting cell growth/metabolite assays, harvest, and cleaning.• Passaged cells for the cell growth and performance studies.• Conducted cell growth/metabolite assays for the samples from Bench top Bioreactors, AMBR15 high-throughput reactors.• Hands-on Experience in analyzing samples in cedex-BioHT, Osmometer.• Sequenced the old batch records data for presenting scientific data using statistical software such as SIMCA, MODDE, JMP for better experiment condition analysis.• Altered the PID controller settings (Delta-v, Sartorius Ambr 15) Softwares under supervision during ongoing process.• Tested the different combinations of transfection reagents ratio for HEK 293 cell line.• Maintained accurate and detailed experimental records in an Electronic Lab Notebook.• Analyzed data and generated technical reports, graphs, SOPs, and internal presentations using Microsoft office (Power presentation, Word, Excel).• Managed lab supplies such as tubing, welders, bioreactor parts and single use equipment by efficiently ordering, stocking, and organizing them.• Ensured a clean and organized laboratory environment.• Prepared reagents, media/buffer solutions, and equipment to facilitate experimental procedures.• Fostered effective verbal communication skills and collaboration with the supervisor and colleagues.• Provided support for additional tasks and projects as needed.

Hyderabad, Telangana, India

• Reviewed general control records, equipment logbook, and batch production records (BPRs).• Worked with 70L, 1200L and 1800L scale fermenters (Biozeen & Sartorius) for execution of fermentation batches.• Performed in-process control tests like OD600 for cell growth and purity by microscopy.• Calibrated the essential Probes (pH, DO, Temp) before the start of the batch.• Monitored process trends such as pH, Temperature, Dissolved Oxygen, and Agitation during fermentation.• Modify changes during the fermentation process in Sartorius MCPS software.

Hyderabad Area, India

• Involved in primary setup, cleaning, sterilization cycles (CIP, SIP, FSIP and Harvest) for fermenters.• Prepared media for growing cells (E. coli and Saccharomyces cerevisiae) in shake flasks and production fermenters (Manual and Automatic systems)• Inoculated shake flasks and fermenters aseptically. • Streamlined setup, operated and troubleshooted fermenters to produce the recombinant protein of interest.• Monitored upstream process parameters (pH, temperature, dissolved oxygen, carbon source) to effectively control cell growth and recombinant protein production.• Optimized fermentation process to increase productivity.• Conducted primary recovery operations using centrifuge to separate cells from the fermentation cell broth.• Operated glass fermenters and stainless-steel fermenter (Winpact and Scigenics) of various sizes (3L, 5L, 10L, 20L & 40L) for batch fermentation and fed-batch fermentation.

Bachelor Of Technology - Btech, Biotechnology

Master Of Science - Ms, Biotechnology