Heather Currie Email & Phone Number

Edinburgh, Scotland, United Kingdom

Edinburgh, Scotland, United Kingdom

Edinburgh, Scotland, United Kingdom

• Quotient － Penicuik, Midlothian Team Lead (Infectious Disease Immunoassay Screening)
• Coordination of extensive assay evaluation to ensure regulatory requirements for sensitivity and specificity could be met.
• Driving completion of documentation including Development Protocols / Reports and review of Validation and Tech Transfer documentation
• Ensuring robust planning and scientific validity of experiments
• Providing project performance and timeline updates (short & long term) to senior management
• Demonstrated consistent hard work to achieve results and improve operations through tools such as Root Cause Analysis and project troubleshooting

Milton Bridge, Midlothian, United Kingdom

• Supervision of small group, providing technical expertise and development opportunities
• Development and Validation of QC methods utilising Affinity and Size Exclusion HPLC methods for antibody characterisation
• Development of purification strategies for blood group antibodies utilising ÄKTA chromatography systems including scale up to pilot level system
• Transfer of methods to ISO13485 manufacturing facility including cross department staff training, and method validation
• Feasibility testing of antigens for use in infectious disease screening immunoassay development
• Production of development and validation protocols/ reports for characterisation and purification methods

Nr Edinburgh

• Worked on the purification of polyclonal antibodies and phage and technically transferred processes into GMP manufacture. Techniques utilised in this work included
• serum precipitation
• affinity chromatography, including preparation and packing of columns
• nanofiltration for viral clearance
• tangential flow filtration and hollow fibre filtration for diafiltration and concentration
• size- exclusion, ion exchange and affinity chromatography using ÄKTA explorer, purifier and pilot systems, and HPLC using Dionex LC systems

Medford, Massachusetts

• Cloned and expressed recombinant proteins in E. coli including batch fermentation and their subsequent chromatographic purification. In addition examined the suitability of recombinant bacterial collagen as a substrate.
• Molecular cloning including gel electrophoresis (DNA and protein), transformation, DNA and RNA isolation
• Western blot, PCR, primer design,.
• Analysis of recombinant proteins by microscopy, SEM and AFM.
• Mammalian cell culture, rt-PCR and quantitative Real-Time PCR.

Clifton Campus, Nottingham

Method development for the characterisation of plant cell wall carbohydrates and proteins. Extraction and analysis of monosaccharides by high pH anion exchange chromatography (HPAEC-PAD) and proteins/ peptides by gas chromatography (GC-FID) and UV spectroscopy. Quantification of trace inorganics by inductively coupled plasma (ICP-AES).