• Directly contributed to the BLA filing (3.2.S.3.1 Elucidation of structure and 3.2.S.3.2 Impurities) of an Antibody candidate. Developed the methods, optimized test conditions, executed protocols, analyzed data, primary author of technical reports, worked collaboratively and maintained timelines. • Developed RP and HILIC methods for characterization of small molecule by positive mode CID and used MS and MSMS information to gain sample specific differences.• Developed Mass spectrometry (Sciex Triple ToF 5600) based Standard Test Methods for the identification and quantification of peptides and phospho-peptides in a vaccine drug product.• Performed characterization of both mono and bi-specific antibodies such as released glycan analysis (Rapifluor), PTM monitoring (relative levels of Met oxidation, Asp isomerization and deamidation, Pyro E, lysine clipping), multiple enzyme peptide and disuldfide bond mapping, intact MS with PNGaseF, Ides and reduction/ non-reduction.• Validated MS based assays for Identification (specificity) according to ICH guidelines• Developed a native SEC-MS method to observe the non-covalent complexing of peptides to protein• Extensive work with PEAKS (Bioinformatic Solutions), Byologic (Protein Metrics), BioPharmaView (Sciex), ChemDraw, MasterControl and Benchling (eLN)• Familiarity with developing and optimizing Octet365 for binding assays • Present in formal and informal meetings within group and with collaborators for effective study design, communication of results and guidance on next steps.

• Drive the development of biomarker and target discovery by MS-based proteomics (LTQ Orbitrap Velos and QExactive) using affinity transmembrane enrichment with quantitative (TMT10) and qualitative proteomics.• Design and analysis of targeted proteomic assays (MRM) on TSQ (Thermo Vantage).• Developed methods for and conducted the medium throughput screening of over 4500 aptamers for binding of endogenous protein targets in relevant biological matrices by affinity pull down.• Target validation by biochemical techniques such as Western blot, ELISAs, FACS, and antibody drug conjugate (ADC) cell based assays. • Demonstrated ability to perform a wide variety of sample preparation methods for cell lines, biofluids, tissue and bacterial proteomes, including top 2-20 protein removal, solubilization by detergent, HPLC fractionation, and concentration prior to MS analysis.• Extensive work in proteomic data analysis using Mascot searches, ISB/SPC Trans Proteomic Pipeline (TPP), SpotFire, JMP (DoE), SkyLine, FlowJo and Excel.• Present in formal and informal meetings within group and with collaborators for effective study design, communication of results and guidance on next steps.

- Mass spectrometry analysis of biological samples for protein complex identification and protein post-translational modification.- Method development on the LTQ-Velos and Orbitrap mass spectrometers (MS) for phospho-proteomics, SILAC and SILAM. Including sample enrichment strategies, optimization of MS parameters and data interpretation.- Daily calibration and routine maintenance on a variety of ion trap MS.

• Mass spectrometry analysis of biological samples for protein complex identification and protein post-translational modification.• Performed daily calibrations and routine maintenance on a variety of ion trap MS.• Highly skilled in tissue preparation and protein enrichment for Activity Based Protein Profiling (ABPP) for poly-acrylamide gel analysis and MS.• Responsible for handling samples in collaboration with outside labs, including tissue culture, inhibitor characterization using competitive ABPP, protein analysis.• Experienced in mammalian tissue culture (SILAC) and sterile technique.• Experienced with aseptic technique for xenograft cancer models in SCID mice.• Small animal handling for tailing, tagging, surgery, and tissue harvesting.• Maintained genotype records of large mouse colonies by Southern blot and PCR.• Managed lab operations including: monthly budgeting plans, ordering supplies, making buffers, production of large poly-acrylamide gels, general lab maintenance.• Served as the lab safety and radiation officer.