Heidi Chial, Ph.D. Email & Phone Number

Denver, Co, Us

Aurora, Co, Us

Work with members of the University of Colorado Alzheimer's and Cognition Center (CUACC), the Department of Neurology, and the Linda Crnic Institute for Down Syndrome to prepare grant applications and manuscripts for publication. Involved in the design, development, and implementation of both basic science and clinical research projects. Co-Director, Grant Writing in Cancer Biology graduate student course (CANB7690).

Aurora, Co, Us

Work with members of the University of Colorado Alzheimer's and Cognition Center (CUACC), the Department of Neurology, and the Linda Crnic Institute for Down Syndrome to prepare grant applications and manuscripts for publication. Involved in the development and implementation of both basic science and clinical research projects. • Associated Publications:- Caneus. J., Granic, A., Rademakers, R., Dickson, D.W, Coughlan, C.M., Chial, H.J., Potter, H. (2017) Mitotic defects lead to neuronal aneuploidy and apoptosis in frontotemporal lobar degeneration caused by MAPT mutations. Molecular Biology of the Cell 2017 Dec 27. pii: mbc.E17-01-0031. doi: 10.1091/mbc.E17-01-0031.- Hamlett, E., Ledreux, A., Potter, H., Chial, H., Espinosa, J., Bettcher, B.M., Granholm, A-C. (2017) Exosomal Biomarkers in Down Syndrome and Alzheimer’s Disease. Special Issue on Down Syndrome, FREE RADICAL BIOLOGY & MEDICINE. pii: S0891-5849(17)30744-X. doi: 10.1016/j.freeradbiomed.2017.08.028.- Caneus, J., Granic, A., Chial, H.J., and Potter, H. (2017) Using Fluorescence In Situ Hybridization (FISH) Analysis to Measure Chromosome Instability and Mosaic Aneuploidy in Neurodegenerative Diseases. In Genomic Mosaicism in Neurons and Other Cell Types. Eds. Jose M. Frade and Fred H. Gage, Neuromethods, Humana Press, 131: 329-359. • Teaching Responsibilities:- Co-Faculty Member, Hypothesis Development and Experimental Design (Cancer Biology 7680), CU-AMC, Cancer Biology Department, Graduate Student Course, Spring 2016, 2017, and 2018.

Aurora, Co, Us

Work with members of the University of Colorado Alzheimer's and Cognition Center (CUACC), the Department of Neurology, and the Linda Crnic Institute for Down Syndrome to prepare grant applications and manuscripts for publication. Involved in the development and implementation of both basic science and clinical research projects.

Aurora, Co, Us

Work with members of the Rocky Mountain Alzheimer's Disease Center and the Linda Crnic Institue for Down Syndrome Research to prepare grant applications and manuscripts.

• Work with Clients to address biomedical writing, editing, educational, and consulting needs. Focused on the development of scientific manuscripts, grants, training grants, white papers, presentations, abstracts, posters, teaching materials, and other technical documents in diverse biomedical and basic science subject areas.

• Provide developmental editing services and write articles for various Nature Education article series.• Coordinate responses to incoming "Ask an Expert" questions from genetics professors/teachers, students (graduate, undergraduate, and high school), and the general public from around the world, which are posted on Scitable.com. Answer questions, manage and recruit Experts, and review/edit answers submitted by Experts.• To date, I have answered more than 200 "Ask an Expert" questions, and I have reviewed/edited more than 200 answers from other Experts.• Authored Spotlight article focused on restriction enzymes.• Technical editor for Scitable Foundations, Essentials of Genetics eBook, which includes articles focused on genetic principles and population genetics. • Authored "Scientists Can Analyze Gene Function by Deleting Gene Sequences" article about how to make a knockout mouse for Scitable Foundations, Essentials of Genetics eBook.

• Edited scientific articles in subject areas including cell biology, biochemistry, molecular biology, genetics, neuroscience, oncology, developmental biology, and medical sciences. • Responsible for more than 100 diverse editing/writing projects.

• Edited, wrote, and reviewed scientific articles, grants, training grants, theses, and other technical documents in diverse biomedical and basic science subject areas.

Washington, Dc, Us

• Procured (prosecuted) patent applications in the fields of recombinant DNA technology, therapeutic proteins, antibodies, oncology, stem cells, bioassays, nanotechnology, and small molecules.• Drafted patent applications (provisional and nonprovisional) and prepared responses to Office Actions. • Provided scientific analysis related to due diligence and opinion letters regarding patentability, patent validity, and freedom to operate.

• Edited scientific articles in subject areas including cell biology, biochemistry, molecular biology, genetics, neuroscience, oncology, developmental biology, and medical sciences. • Lead Writer (Genes and Disease Topic Room), Writer (Genomics Topic Room) for Scitable.com (Nature Publishing Group, Nature Education). Authored 16 articles, managed the work of 3 writers.• Associated Publications: -Chial, H. (2008). Cytogenetic Methods in Diagnosing Genetic Disorders. Nature Education 1(1).-Chial, H. (2008). Polygenic Inheritance and Gene Mapping. Nature Education 1(1).-Chial, H. (2008). Mendelian Genetics: Patterns of Inheritance and Single-Gene Disorders. Nature Education 1(1).-Chial, H. (2008). Proto-Oncogenes to Oncogenes to Cancer. Nature Education 1(1).-Chial, H. (2008). Tumor Suppressor (TS) Genes and the Two-Hit Hypothesis. Nature Education 1(1).-Chial, H. (2008). Gene Mapping and Disease. Nature Education 1(1).-Chial, H. (2008). Huntington's Disease: The Discovery of the Huntingtin Gene. Nature Education 1(1).-Chial, H. (2008). Somatic Mosaicism and Chromosomal Disorders. Nature Education 1(1).-Chial, H. (2008). DNA Sequencing Technologies Key to the Human Genome Project. Nature Education 1(1).-Chial, H. (2008). Rare Genetic Disorders: Learning About Genetic Disease Through Gene Mapping, SNPs, and Microarray Data. Nature Education 1(1). -Chial, H. (2008). Genetic Regulation of Cancer. Nature Education 1(1). -Chial, H. (2008). DNA Fingerprinting Using Amplified Fragment Length Polymorphisms (AFLP): No Genome Sequence Required. Nature Education 1(1).-Chial, H. (2008). Cytogenetic Methods and Disease: Flow Cytometry, CGH and FISH. Nature Education 1(1). -Chial, H. (2008). Gene-Based Therapeutic Approaches. Nature Education 1(1).-Chial, H. and Craig, J. (2008). Genome-Wide Association Studies (GWAS) and Obesity. Nature Education 1(1).-Chial, H. and Craig, J. (2008). mtDNA and Mitochondrial Diseases. Nature Education 1(1).

Palo Alto, Ca, Us

• Wrote grant applications (NIH and private) and manuscripts, carried out experiments, and developed an NIH NRSA-funded research program focused on the functional characterization of APPL1 and APPL2 proteins in neurotrophin-mediated signaling pathways and links to neurodegenerative disease.• Associated Publication:-Chial, H. J., Wu, R., Ustach, C., McPhail, L. C., Mobley, W. C., and Chen, Y. Q. (2008). Membrane Targeting by APPL1 and APPL2: Dynamic Scaffolds that Oligomerize and Bind Phosphoinositides. Traffic 9: 215-229.

Winston-Salem, Nc, Us

• Wrote grant applications (NIH) and manuscripts, carried out experiments, and developed an NIH NRSA-funded research program related to the functional characterization of human APPL1 and APPL2 BAR, PH, and PTB domains (phosphoinositide binding, membrane targeting, and protein-protein interactions).• Co-Faculty Member (Cell Biology of Cancer Graduate Student Course, CanBio704, Spring 2005 and 2006).• Faculty Course Facilitator (Cancer Biology Journal Club, CanBio711, Fall 2004, Spring 2005, and Fall 2005).• Course Lecturer (Topic: FRET Microscopy, Microscopic Research Techniques Graduate Student Course, PATH 719, Spring 2006).•Associated Publications:-Chial, H. J., Lenart, P., and Chen, Y. Q. (2010). APPL Proteins FRET at the BAR: Direct Observation of APPL1 and APPL2 BAR Domain-Mediated Interactions on Cell Membranes Using FRET Microscopy. PLoS ONE 5(8): e12471.-Chial, H. J., Wu, R., Ustach, C., McPhail, L. C., Mobley, W. C., and Chen, Y. Q. (2008). Membrane Targeting by APPL1 and APPL2: Dynamic Scaffolds that Oligomerize and Bind Phosphoinositides. Traffic 9: 215-229.

Woods Hole, Ma, Us

• I was a postdoctoral student in the MBL Summer Neurobiology Course, which is an intensive nine-week course consisting of electrophysiology, imaging, and molecular sections. This course included 150 hours of lectures and over 400 hours of laboratory training in key concepts and techniques important for neurobiology research from leading experts.• I received a post-course research scholarship to carry out a research project focused on FRET analysis of APPL BAR domain protein-protein interactions.• Associated Publication:Chial, H. J., Lenart, P., and Chen, Y. Q. (2010). APPL Proteins FRET at the BAR: Direct Observation of APPL1 and APPL2 BAR Domain-Mediated Interactions on Cell Membranes Using FRET Microscopy. PLoS ONE 5(8): e12471.

Analyzed, summarized, and curated biological, genomic, and proteomic information for the Mammalian BioKnowledge Library (BKL) Proteome Database.

Northfield, Mn, Us

• As an Assistant Professor, I taught the following undergraduate courses:-Cellular Biology and Genetics (Bio125), Fall 1999 and Fall 2000-Genetics, Evolution, and Society (Bio127), January Term 2000 -Elementary Bio-Organic Chemistry (Chem122), Spring 2000-Intermediate Genetics (Bio233), Spring 2001• Taught lecture and laboratory components for all courses (up to 65 students), prepared all lectures using PowerPoint slide shows and posted to course websites.• Responsible for all grading, designed lab exercises, and wrote lab manuals.• Academic advisor for 30 students each semester (2000-2001).

Rochester, Minnesota, Us

Carried out experiments focused on the human BRCA2 breast cancer gene and the yeast homolog of the human frataxin gene, which is associated with Friedreich ataxia.

Boulder, Colorado, Us

• Ph.D. Thesis: Analysis of S. cerevisiae NDC1 reveals a link between spindle pole bodies and nuclear pore complexes. • These studies: 1) demonstrated a shared localization of yeast Ndc1p to spindle poles and nuclear pore complexes, 2) identified and characterized genetic interactions between NDC1, EAP1, and POM152, and 3) uncovered NDC1 gene dosage effects that lead to genetic instability phenotypes in yeast.• Teaching Assistant for Principles of Genetics (MCDB 2150) and Molecular Cell Biology (MCDB 3135) undergraduate lecture and laboratory courses.• Associated Publications:-Chial, H. J., Stemm-Wolf, A. J., McBratney, S., and Winey, M. (2000). Yeast Eap1p, an eIF4E-Associated Protein, has a Separate Function Involving Spindle Pole Bodies and Genetic Stability. Current Biology 10: 1519-1522.-Chial, H. J. and Winey, M. (1999). Mechanisms of genetic instability revealed by analysis of yeast spindle pole body duplication. Biology of the Cell 91: 439-450.-Chial, H. J., Giddings, T. H. Jr., Siewert, E. A., Hoyt, M. A., and Winey, M. (1999). Altered Dosage of the Saccharomyces cerevisiae Spindle Pole Body Duplication Gene, NDC1, Leads to Aneuploidy and Polyploidy. Proceedings of the National Academy of Sciences 96: 10200-10205.-Chial, H. J., Rout, M. P., Giddings, T. H., and Winey, M. (1998). Saccharomyces cerevisiae Ndc1p is a Shared Component of Nuclear Pore Complexes and Spindle Pole Bodies. Journal of Cell Biology 143: 1789-1800.

Saint Peter, Mn, Us

• Investigated protein binding properties of Coomassie Brilliant Blue dye.• Chemistry Department Academic Assistant (1992-1993). • Teaching Activities: Inorganic Chemistry Lab Teaching Assistant (Spring 1993), Biochemistry I Lab Teaching Assistant (Fall 1992), General Chemistry Tutor (Fall 1992), Inorganic Chemistry Lab Teaching Assistant (Spring 1992), Inorganic Chemistry Tutor (Spring 1992), Biochemistry Tutor (Spring 1992), Biochemistry Lab Teaching Assistant (January Term 1992), Organic Chemistry II Tutor and Grader (Fall 1991), General Chemistry Lab Teaching Assistant (Fall 1991).• Associated Publications:-Chial, H. J., Congdon, R. W., and Splittgerber, A. G. (1995). A Ligand Binding Assay Based on a Protein Assay Method. Journal of Chemical Education 72: 76-79.-Chial, H. J. and Splittgerber, A. G. (1993). A Comparison of the Binding of Coomassie Brilliant Blue to Proteins at Low and Neutral pH. Analytical Biochemistry 213: 362-369.-Chial, H. J., Thompson, H. B., and Splittgerber, A. G. (1993). A Spectral Study of the Charge Forms of Coomassie Brilliant Blue G. Analytical Biochemistry 209: 258-266.

Postdoctoral Research Scholar, Dept. Neurology And Neurological Sciences, Neuroscience Institute At Stanford

Post-Doctoral Research Fellow, Dept. Cancer Biology

Postdoctoral Student, Mbl Summer Neurobiology Course

Post-Doctoral Research Fellow, Depts. Biochemistry And Molecular Biology, Pediatric And Adolescent Medicine

Ph.D., Dept. Molecular, Cellular, And Developmental Biology

B.A., Chemistry, Biochemistry And Molecular Biology, Magna Cum Laude