Heidi Burke Email & Phone Number

Brentford, Middlesex, GB

Leading the Molecular Biology MAPS team

Cambridge, Massachusetts, US

Cambridge, Massachusetts, US

Head of Protein Science in Discovery department. Focused on structure-based design of protein antigenic constructs and leading team in protein production and MAPS assembly for the generation of our discovery vaccine candidates.

Paris, France, FR

Expressed, purified, crystallized and solved the structure of an antibody used in tri-specific therapeutics.Engineering constructs of an antigen that is the target of antibody therapeutics. Optimized expression, proteolysis and purification. Form antibody/antigen complexes and screened for crystallization and cryo-EM

Medford, Massachusetts, US

• Engineered a soluble, crystallizing version of HCMV gB and used X-ray crystallography to determine the atomic structure, gaining extensive experience in bacterial and insect (SF9 and S2) protein expression and.
• Established collaborations and procured HCMV positive human sera and human anti-gB monoclonal antibodies to test for a correlation between antibody binding of specific gB domains and protection from disease in neonates.
• Trained and supervised a rotation student and instructed medical/dental students in microbiological techniques.
• Member of the Tufts Biomedical Business Club and a Graduate Student Council Representative responsible for organizing graduate student events, including fundraising for the student enrichment fund.
• Molecular Microbiology - MERGE-ID track. Laboratory of Dr. Katya Heldwein

Boston, MA, US

• While in the laboratory of Dr, Samuel M. Moskowitz at MGH:
• Demonstrated LpxO2 of Pseudomonas aeruginosa, (P. aeruginosa) increases polymixin resistance in a ΔpmrAB or ΔphoPQ background using genetics, antimicrobial susceptibility testing, and MALDI-TOF MS.
• Mentored a student and trained three scientists to perform zebrafish injections and other laboratory techniques.
• Transferred the laboratory from Seattle, acquiring, setting up and organizing all equipment and supplies. Wrote animal use protocols. Established laboratory practices and cultivated a new culture.

Seattle, WA, US

• While in the laboratory of Dr. Samuel M. Moskowitz at the University of Washington:
• Under the direction of Dr, S. M. Moskowitz, Investigated the role of the two-component systems, PhoPQ and PmrAB, in P. aeruginosa virulence using zebrafish embryos as a model for innate immunity-pathogen interaction.
• Analyzed genomic sequences to implicate factors that may confer antibiotic resistance in P. aeruginosa and used microbiological techniques to validate them to improve treatment of infections in Cystic Fibrosis (CF).
• Established an extensive library of P. aeruginosa CF clinical strains, cataloging, speciating, and sequencing each.

Seattle, WA, US

• While in the Laboratory of Dr. A. R. La Spada:
• Established a triple transgenic murine line.
• Determined if developmental disabilities seen with Bardet–Biedl syndrome (Bbs4) are due to the presence of cortex ubiquitinated protein inclusions using immunocytochemistry on cryosections of a Bbs4 murine model.
• Performed behavioral studies on several murine lines to assist in various neurodegenerative disease research projects.

Doctor Of Philosophy (Ph.D.), Molecular Microbiology - Merge-Id Track With A Structural Biology Focus

Bachelor'S, Biochemistry

Bachelor'S Degree, Biochemistry