• Help develop drafts of posters, abstracts and PLSs • Took minutes at meeting with clients• Took in internal and external reviewers’ comments into work• Represented team at meetings with clients• Data checking of colleagues’ work

• Help write/edit SOPs • Record keeping (Lab Books – One Note)• Tested oncolytic viruses on a selection of different cancer types and normal cell types to determine killing and replication ability• Help prepare patient blood sample for testing• Represented lab group at meetings with internal and external collaborators

Carried out experiments for several ongoing projects.Represented lab group at meetings with internal and external collaborators.Lab management: purchasing materials coordinating the shipment drugs to be tested.Developed skills in early drug development (early biological testing phase).Developed skills such as CRISPR.

Maintenance of cell lines for the Pharmacology & Drug Development GroupCarried out collaborative experiments for ongoing lab projects Effective lab management: purchasing materials, maintaining records of chemicals, orders and cell lines, updating SOPs, co-ordinating with Cell Bank for the revival/banking of cells in liquid nitrogen, mycoplasma and bacterial testing, etc.Represented lab group at several multi-team meetings (Floor, Cell Bank, etc.)Developed skills in preparing cells for in vivo tumour implantation move upCo-ordinated with colleagues/other universities to cells to external labs

Papers Published:McCann, Henry, Johnson, Charlotte, Errington, Rachel, Davies, D. Mark, Dunlop, Elaine and Tee, Andrew 2018. Energy stress-mediated cytotoxicity in tuberous sclerosis complex 2-deficient cells with nelfinavir and mefloquine treatment. Cancers 10 (10), 375.Johnson, Charlotte E., Dunlop, Elaine A., Seifan, Sara, McCann, Henry D., Hay, Trevor, Parfitt, Geraint J., Jones, Ashley T., Giles, Peter J., Shen, Ming H., Sampson, Julian R., Errington, Rachel J., Davies, David Mark and Tee, Andrew R. 2018. Loss of tuberous sclerosis complex 2 sensitizes tumours to nelfinavir−bortezomib therapy to intensify endoplasmic reticulum stress-induced cell death. Oncogene 37, pp. 5913-5925

Sorted samples received from GPs, and from within the hospital.Ensured that samples and corresponding forms matched and were sent to correct laboratories. Investigated mislabelled samples/formsInput Patient details in computer systemSorted and mailed lab reports to respective Doctors

Project - "Purification of histamine decarboxylase"Supervisor - Dr. Eoin FlemingPart of the UCC Ureka projectTechniques used,Bacterial Cell culturingProtein purification Western Blotting