-Synthesized and purified a form of Ribose Binding Protein that uses a Red-Green FRET sensor to quantify the amount of ribose in a sample-Utilized gel electrophoresis, spectrofluorometry, and ITC to determine the purity, strength of the FRET signal, and binding coefficient of the protein in vitro-Imaged cells containing the protein using a confocal microscope and wrote a MATLAB script to calculate the FRET signal in vivo from the images-Developed a MATLAB script to remove noise from… Show more -Synthesized and purified a form of Ribose Binding Protein that uses a Red-Green FRET sensor to quantify the amount of ribose in a sample-Utilized gel electrophoresis, spectrofluorometry, and ITC to determine the purity, strength of the FRET signal, and binding coefficient of the protein in vitro-Imaged cells containing the protein using a confocal microscope and wrote a MATLAB script to calculate the FRET signal in vivo from the images-Developed a MATLAB script to remove noise from images of cells, improving the accuracy of pixel-by-pixel calculations Show less