Erik Holtzapple

Erik Holtzapple Email and Phone Number

Director of Research at Provenance Bio @ Debut Biotechnology
San Diego, CA, US
Erik Holtzapple's Location
San Diego, California, United States, United States
Erik Holtzapple's Contact Details

Erik Holtzapple personal email

Erik Holtzapple phone numbers

About Erik Holtzapple

A scientist with over 15 years of industrial experience in enzyme assays, strain/ host engineering, protein expression, and gene discovery in both bacterial systems (E.coli, Bacillus, cyanobacteria, and methanotrophs) and eukaryotic systems (algae and yeast/ fungal systems). Research areas include strain engineering/development, metabolic engineering, gene design, and gene discovery using genomic information, the most current molecular biology techniques, and biochemical methods. Expertise in enzymology with enzyme kinetics and assay development. A problem solver, creative, and adaptable; allowing achievement of results quickly. Successfully managed and mentored junior scientists at the bench. Proficient in coordinating and managing projects and effectively communicating in multidisciplinary teams.Specialties: biocatalysis, enzyme assays, molecular biology (PCR, error prone PCR, cloning, gDNA library construction, etc.), Western blotting, antisense RNA, RNAi, qRT-PCR, gene/promoter reporter assays, enzyme kinetics, analytical chemistry (HPLC, GC, MS, thin layer chromatograghy), FPLC (protein purification).Computer skills: Excel, Chemdraw, Unix, SQL, Vector NTI, Sequencher, CLC, and other bioinformatic tools (BLAST, NCBI, EcoCyc)

Erik Holtzapple's Current Company Details
Debut Biotechnology

Debut Biotechnology

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Director of Research at Provenance Bio
San Diego, CA, US
Erik Holtzapple Work Experience Details
  • Debut Biotechnology
    Debut Biotechnology
    San Diego, Ca, Us
  • Provenance Bio
    Director Of Research
    Provenance Bio Aug 2022 - Present
    San Francisco, Ca, Us
  • Debut Biotechnology
    Senior Scientist
    Debut Biotechnology Oct 2020 - Jun 2022
    San Diego, California, Us
  • Genomatica
    Senior Research Scientist
    Genomatica Jun 2019 - Sep 2020
    San Diego, Ca, Us
  • Nucelis Llc
    Strain Development Senior Scientist
    Nucelis Llc Oct 2015 - Jun 2019
    San Diego, Ca, Us
    Strain development of non-conventional yeasts and bacteria to produce natural products using synthetic biology and genome editing methods.
  • University Of California San Diego
    Senior Research Associate Ii
    University Of California San Diego Jan 2015 - Sep 2015
    La Jolla, Ca, Us
    Designed "wetlab"experiments to confirm datasets of E.coli protein-protein interactions ( termed the "interactome") from yeast two hybrid experiments. I successfully identified several novel protein-protein interactions that were previously uncharacterized. https://www.nature.com/articles/nbt.4024
  • Intrexon Corporation
    Senior Scientist
    Intrexon Corporation May 2013 - Aug 2014
    Germantown, Maryland, Us
    Tool development in non-conventional methanotrophs. Promoter characterization in methanotroph systems.
  • Synthetic Genomics
    Scientist Ii
    Synthetic Genomics Nov 2009 - Oct 2012
    La Jolla, Ca, Us
    •Created and validated novel lipogenic pathways in cyanobacteria species for enhanced biofuel productivity•Utilized gDNA libraries for identifying novel efflux pumps in E.coli using HTP colorimetric screens. • Led a gene validation research team characterizing metabolic pathways associated with neutral lipid accumulation in eukaryotic algal strains • Developed enzymatic assays for characterizing heterologously expressed enzymes in cyanobacteria and eukaryotic algae• Utilized RNAi /antisense RNA for knockdown of native competing metabolic pathways in eukaryotic algal strains.
  • Agraquest
    Scientist I- Molecular Biologist
    Agraquest Jun 2008 - Nov 2009
    • First hired Molecular Biologist established molecular biology platform tools for genetically manipulating industrial Bacillus strains• Designed in vitro anti-fungal synergy assays using natural products produced by Bacillus strains• Drove genome sequencing and analysis of several AgraQuest strains• Provided molecular biology support (i.e. training personnel)
  • University Of Minnesota
    Graduate Student
    University Of Minnesota Aug 2002 - Jul 2008
    Minneapolis And St. Paul, Minnesota, Us
    For my PhD thesis, I characterized two acyl-transferases and an acyl-CoA synthetase from the hydrocarbon-degrading marine bacteria Marinobacter hydrocarbonoclasticus DSM 8798. I cloned several candidate acyltransferase genes as well as several acyl-CoA synthetases (acyl-CoA ligases) using Marinobacter aqualeoi VT8 genome sequence. The enzymes were characterized using in vitro coupling assays that generated acyl and isoprenoid wax ester products.
  • The Institute For Genomic Research
    Research Associate Iii
    The Institute For Genomic Research Oct 1997 - Aug 2002
    Microbial Genomics Supervisor: Timothy Read, PhD• Assisted in the DNA sequence assembly of several microbial genomes (Streptococcus pneumoniae, Bacillus anthracis,Chlamydophila caviae, and Pseudomonas putida)• molecular biology techniques - cloning, PCR, dye terminator sequencing• bioinformatics and computational biology techniques (UNIX, SQL, PERL) to aide in the closure of these genomes.

Erik Holtzapple Skills

Molecular Biology Protein Purification Microbiology Protein Chemistry Biochemistry Genomics Pcr Assay Development Dna Enzyme Kinetics Protein Expression Enzyme Assays Biotechnology Western Blotting Bioinformatics Hplc Biofuels Metabolic Engineering Computational Biology Polymerase Chain Reaction Sequencing Fplc Rnai Sds Page Gel Electrophoresis Strain Development Gene Discovery Fermentation Qpcr In Vitro Purification

Erik Holtzapple Education Details

  • University Of Minnesota
    University Of Minnesota
    Biochemistry
  • The Johns Hopkins University
    The Johns Hopkins University
    Biotechnology
  • Clemson University
    Clemson University
    Microbiology

Frequently Asked Questions about Erik Holtzapple

What company does Erik Holtzapple work for?

Erik Holtzapple works for Debut Biotechnology

What is Erik Holtzapple's role at the current company?

Erik Holtzapple's current role is Director of Research at Provenance Bio.

What is Erik Holtzapple's email address?

Erik Holtzapple's email address is eh****@****ail.com

What is Erik Holtzapple's direct phone number?

Erik Holtzapple's direct phone number is +161270*****

What schools did Erik Holtzapple attend?

Erik Holtzapple attended University Of Minnesota, The Johns Hopkins University, Clemson University.

What are some of Erik Holtzapple's interests?

Erik Holtzapple has interest in Surfing, Drawing.

What skills is Erik Holtzapple known for?

Erik Holtzapple has skills like Molecular Biology, Protein Purification, Microbiology, Protein Chemistry, Biochemistry, Genomics, Pcr, Assay Development, Dna, Enzyme Kinetics, Protein Expression, Enzyme Assays.

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