H V Sudheendra Email & Phone Number

Bengaluru, Karnataka, India

• Molecular gene editing projects:o Assumed a leadership role with 3-4 direct reports, while actively contributing as a team member. Provided guidance by teaching specialized techniques, organizing tasks, and overseeing.
• Immuno-oncology projects: o Optimized immuno-oncology assays for large molecule screening and assessed numerous bi-specific and tri-specific antibodies.o Conducted binding and cytotoxicity assays with mentioned.

Bengaluru Area, India

• Immuno-oncology projects: o Optimized T-cell activation assays for various approaches like T & dendritic cells co-culture, PBMC stimulation assay by external peptide addition, & Synthetic Antigen Presenting cells.o.
• Aplastic anemia project – Whole Exome Sequencing of over 48 samples were done, data analyzed; gene expression profiling of novel & known mutations by RT-PCR was done, and a correlation with TERT expression pattern was.
• Validated 70 cervical cancer samples by RT-PCR for new & known mutations.
• Processed 50 primary tumor tissues from Breast cancer, cervical cancer and head and neck cancer and established at least 15 primary cell lines for research purposes.
• Maintained, expanded, and harvested multiple cancer cell lines for use in NGS applications as a control sample.
• Managing multiple projects and delivering the results on time and mentoring the junior lab members.

Bengaluru Area, India

• “Salivary transcriptome & proteome for the diagnosis & prognosis of oral cancer”
• Processed saliva samples by iTRAQ method for Mass spectrometric analysis.
• Performed statistical data cleanup using “Genespring” software.
• Combined the data from biological replicates, analyzed and made interpretations.
• Validated the samples for potential biomarkers by Western Blot & ELISA and co-authored the publication.“Molecular signatures specifying cancer stem cell-like cells from oral squamous cell carcinoma and their relevance.
• Generated & cultured drug resistant cells for in vitro & in vivo studies.

Yelahanka, Bengaluru

• Taught MSc, MPhil, and Diploma in Regenerative Medicine studentso Trained the them for isolation, culturing, characterization & differentiation of stem cells from various human and murine sources.o Organized &.
• R&D activities as a part of ‘Cancer Stem Cell’ groupo Isolated and cultured tumor cells, cancer stem cells, & stem cells from various tissue sources and characterized by RT-PCR, Flowcytometry & trilineage.

Bangalore

• Handled both theory and practical classes for bachelor’s in Biotechnology students.
• Helped setting up a fully functional biotechnology lab.

Master Of Science (M.Sc.), Biotechnology

Bachelor Of Science (B.Sc.), Biotechnology, Chemistry & Applied Botany