Hwoi Chan Kwon Email & Phone Number

Bethesda, Md, Us

Bethesda, Maryland, United States

Instructed over 100 undergraduate students in biochemistry fundamentals, teaching theoretical laboratory techniques such as column chromatography and gel electrophoresisStrategically aligned teaching methods with core biochemistry concepts on structure, function, and metabolism, leading to an increase in performance metrics.Facilitated engaging discussions and developed comprehensive supplementary materials, increasing student engagement.

Assisted in preparing and submitting Reports of Invention (ROI) for UC Davis Innovation Access to facilitate commercialization processesConducted comprehensive technical research to assess potential conflicting inventions; identified possible overlaps in intellectual property that may lead to USPTO patent submission rejection. Drafted non-confidential marketing and licensing materials; communicated the value proposition of inventions to potential partners and investors

Davis

Generated novel mutations using recombinant DNA technology by designing primer oligo for site-directed mutagenesis, performing polymerase chain reaction, transforming E.Coli using heat-shock and electroporation, amplifying plasmid vector, mini prepping plasmid, and quantifying nucleic acid using UV-Vis spectroscopy.Experienced in culturing, harvesting, and prepping various expression systems ranging from adherent mammalian cell lines, such as HEK293 and CHO to X. laevis oocytes. Performed lipid-based transient transfection for heterologous expression in the mammalian cell line within the guidelines of a biosafety level 2 (BSL-2) environment; performed manipulation and injection of X. oocytes with mRNA load using the nanoliter injection system.Identified and quantified heterologous expression and activity of recombinant protein using wide-field fluorescence, total internal reflection fluorescence (TIRF), and fluorometry.Designed and performed patch-clamp electrophysiology using pCLAMP to generate thousands of high-quality recording traces using voltage and current clamp protocols, leading to the discovery of a novel gating mechanism for a CLC channel.Performed and analyzed two-electrode voltage clamp (TEVC) and single-channel recordings in quantifying macroscopic and microscopic properties of ion channels. Analyzed electrophysiology data using regression and statistical methods on Clampfit, excel, and Originlab (analogous to Graphed).Wrote technical papers and reviews and collaborated on original research, as demonstrated by first and second author publications.

Doctor Of Philosophy (Phd), Biophysics

Bachelor Of Science (B.S.), Biochemistry