Molecular biologist with extensive laboratory experience, particularly in nucleic acid based protocols – DNA/RNA extraction, RT-qPCR, SNP genotyping and DNA sequencing using both Sanger and high-throughput sequencing (NGS). Well equipped with key sequencing and variants analysis softwares e.g. SeqScape, SVS and CLC. Worked in implementing and validating NGS-based gene panel for clinical diagnostic purposes e.g Marfan and related disorders. Capable of running gene sequencing assays of BRCA1, BRCA2 and HNPPC using Sanger sequencing. Interested in studying methylome and the role of of DNA methylation changes in the onset of liver fibrosis. Highly motivated, with well-developed communication and a strong team spirit. Specialties: - Running Ion PGM sequencer - Sanger sequencing- Sequence analysis using CLC /SeqScape/SVS- NGS-based library preparation- SNP genotyping- Fluorescence In Situ Hybridization (FISH)- Quantitative real-time PCR (qPCR) - Southern, Northern Blotting- Immunoblotting (Western blot)- Chromatin Immunoprecipitation (ChIP)- RNA interference mediated silencing- Gain–and loss-of-function techniques- Detection and phenotypic analysis of transgenic plants- Stable Isotope Probing (SIP)- SDS-PAGE- Recombinant protein expression and purification- Light and fluorescence microscopy- Microautoradiography (MAR)