4+ years of drug discovery and R&D in a biotechnology start-up:• Platform development. Effective management of multiple projects with competing priorities and deadlines.• Antibody drug discovery with expertise in multiple therapeutic areas.• In-depth knowledge of cell biology, signaling, mouse physiology etc. Co-author of multiple publications and patents. • Demonstrated strong leadership skills and empathy with colleagues.• Proactive, enthusiastic, creative, and results-oriented person with entrepreneurial spirit.• Effective organizational skills with ability to interact with people of different backgrounds and seniority levels. Skills:Antibody R&D: Recombinant monoclonal antibody generation and characterization: design, preparation, expression and purification of antigen; production, purification and evaluation of antibodies for in vitro and in vivo studies. Antibody conjugation and labeling. Antibody binding assays including ligand/receptor and antigen/antibody. Competition assays. Design and generation of DNA expression constructs for production of membrane-anchored antigens. Antibody Phage Display. Antibody cloning and engineering. Affinity maturation of antibodies. EC50/IC50. Graph Pad Prizm and Excel.Cell Biology: cell culture, stable cell line generation, flow cytometry. Direct and indirect immunofluorescence.Cell-based Assays: Cell proliferation, cytotoxicity, inhibition/activation and binding assays. Protein Biochemistry: expression, isolation and purification of proteins, protein labeling and modifications. Molecular Biology: qPCR, cloning, isolation of DNA/RNA, shRNA; protein expression and analysis including Western blot, ELISA, IP. ChIP-seq. Ion torrent and Fluidigm platforms.Mouse Physiologic studies: handling, breeding, animal bleeding, metabolic experiments; isolation and analysis of mouse tissues.Novel method development: Multiplexed signaling pathway analysis using DNA barcodes and NGS.Patent search and writing.