• Extensive experience in preparative chromatography: Purity upgrade, impurity isolation at µg level, desalting, modeling productivity (IEX and RPLC). • Method development: Subject-Matter Expert in developing analytical methods. Built workflows for chiral and achiral liquid chromatography methods for pharmaceutical analysis including buffer selection, column screening and selection, method optimization using software-assisted method development (ACD/Labs), and robustness evaluation, and method transfer.• Software-assisted method development: introduced computer assisted modeling using innovative tools (such as multi-peak cross over modeling) to optimize (chiral-RPLC, achiral-RPLC, HIC, IEX, 2D-LC, etc.). Extended the reach of modeling to complex modalities (proteins, mAbs, vaccines, etc.). Published multiple A Chem papers on this topic. • Two-dimensional liquid chromatography (2D-LC): 1) developed carbon-based stationary phase of unique selectivity for use in 2D-LC (A Chem paper), 2) developed workflows for multi-column 2D-LC screening at analytical Scale (J. Chrom A publication), 3) introduced 2D-LC with enrichment capabilities for impurity isolation at ug level, 4) introduced modeling of both dimensions in 2D-LC (two Analytical Chemistry papers published). Used 2D-LC to enable purifications, method development, mass balance investigation, MS determination with unfriendly buffers, column selection, investigations, audits, and troubleshooting. • In-depth knowledge and hands-on experience in HPLC Troubleshooting (published an invited review article in Chromatographia) and taught full-day short course on HPLC troubleshooting internally at Merck, Novartis and externally at EAS and Chromatography Forum of Delaware Valley. • Established methods and algorithms for detection and quantitation of compounds with poor or no chromophore using charged Aerosol Detection (published five papers on this topic). • Extensive experience in State-of-the-art UHPLC Instrument evaluation from ThermoFisher, Agilent, Waters, and Agilent (published a review article comparing and evaluating the performance of these UHPLC systems).• Developed analytical methods for various stages of drug development (discovery to post approval). GMP experience includes validation of early phase and late phase methods along with supporting analytical packages for NDA submissions. • Established workflows for polymer and copolymer analysis using multi-detection systems composed of HPLC connected to refractometer, light scattering, viscometer, and UV (six publications on this topic).