• Assisted senior scientists in executing protocols to produce new COVID-19 diagnostic and cancer therapeutic monoclonal antibodies• Utilized phage display technology to evaluate antibody binding capacity and viability (electroporation, panning)• Responsible for performing antibody cloning (PCR, transformation, mini-prep/midi-prep, DNA sequencing), production (transient transfection, protein purification), and characterization (SDS-PAGE, ELISA) to identify potential targets to be passed onto other departments for further testing and evaluation• Analyzed, interpreted, and documented data from each experiment in an electronic notebook (Benchling) and presented findings in weekly group meetings• Tracked inventory and ordered reagents and supplies

• Collaborated with other research scientists in examining the alx gene (a manganese transporter) and if specific components were necessary for its function• Performed site-directed mutagenesis on various transmembrane residues to determine localization and effect on gene functionality (overlap extension PCR, agarose gel electrophoresis, DNA sequencing, gel purification, plasmid DNA isolation, DNA quantification) • Utilized molecular cloning techniques to verify successful uptake of plasmid in E. coli cells (bacterial culturing, restriction enzyme digest, DNA ligation, transformation, LB plate preparation and plating) • Verified localization and reactivity of mutated residues via SDS-PAGE and western blotting

• Assisted in processing and shelving new incoming journals, newspapers, and microfilm • Assisted in collection maintenance including weeding and recycling newspapers • Regularly checked and maintained new journal and newspaper displays • Performed re-shelving and shelf maintenance including reporting late newspapers and journals, correcting/reporting overflow, and making missing labels

• Organized and shelved periodicals, newspapers, microfilm, microfiche, and microform using Library of Congress classification• Provided basic instruction in the use of various online catalogs and research databases including Roger, Circuit, Melvyl, Academic Search Complete, and Newsbank • Assisted patrons in locating periodicals, newspapers, reference materials, government documents, and microforms • Provided basic instruction and assistance with use of microform readers/printers and scanners, including changing lenses and changing microfilm/fiche carriages

• Checked in books using book sorter and manually• Organized books onto carts and shelved carts• Shelved books using the Dewey Decimal system• Assisted patrons in finding books