Graduate Student Researcher
Current•I utilized molecular biology techniques such as polymerase chain reaction (PCR), molecular cloning, and protein/DNA electrophoresis to visualize and amplify genes, as well as to modify and analyze genes. •Generated conclusions, judgements, and laboratory reports based on scientific processes and experiment results.•Designed an ELISA based test kit for a fast, specific, and sensitive diagnosis of cat scratch disease.•Proficient in utilizing cell culture techniques for the growth, maintenance, and manipulation of various cell lines.•Experienced in utilizing aseptic techniques, incubators, laminar flow hoods, and other laboratory equipment related to cell culture.•Proficient in the design and optimisation of experiments with qRT-PCR, which encompasses the development of primers for target gene amplification.•Successfully analysed gene expression using qRT-PCR) to examine the expression levels of miR299-5p following treatment with paclitaxel in A549 lung cancer cells.•Successfully designed and executed qRT-PCR experiments, ensuring accurate and reproducible results.•Proficiently handled RNA extraction, cDNA synthesis, and qRT-PCR assays.•The qRT-PCR data was analysed statistically using the 2-ΔΔCT method. The results were interpreted to provide support for the research conclusions.•Familiar with flow cytometry applications including cell cycle analysis, apoptosis detection, intracellular staining, and fluorescence-activated cell sorting (FACS).•Proficient in microbiology culturing techniques, including aseptic handling, isolation, and maintenance of bacterial cultures.•Proficient in performing antimicrobial susceptibility testing, such as disc diffusion and broth dilution methods, to determine bacterial sensitivity to antibiotics.•Experienced in performing biochemical tests, such as catalase, oxidase, and coagulase tests, to identify and differentiate bacterial species.
