Inge Janssen Email & Phone Number

Sittard, Li, Nl

Beek, Limburg, Nederland

Beek, Limburg, Nederland

Geleen

• Protein expression by means of bacterial transformation and cultivation• Protein characterisation and purification (downstream processing)• Genetic engineering and cloning of plasmids• Establishing a quality system for our Production Facility according to GMP and ISO 9001 regulations (QA and QC)• Responsible for equipment maintenance • Responsible for emergency response and health & safety within the company, by working as an Emergency Response Officer and Prevention Officer• Responsible for chemical inventory and exposure & risk assessment of hazardous substances • Gained knowledge of the principles of Lean and Lean Six Sigma, the DMAIC process and tools• Knowledge of Quality Management and Continuous Improvement by using the Deming PDCA-cycle• Supervising interns in the labAbout EnzyPep B.V.EnzyPep B.V. is an innovation-driven company that develops new, sustainable, green, and cost-effective synthetic routes for the production of pharmaceutically relevant polypeptides and conjugates. EnzyPep’s core technology, chemo-enzymatic peptide synthesis or CEPS, is based on a unique combination of classical peptide chemistry (solution- and solid-phase synthesis of peptide segments) and biocatalysis. Our enzyme-catalyzed ligation reactions require unprotected peptide segments and are performed in water. EnzyPep B.V. continually develops and expands its proprietary enzyme platform that is used for the process design and production of marketed peptide APIs.

Geleen

• Cultivation of different anaerobic bacteria strains on sugars and CO2, and synthesis gas (CO/H2)• Transformation of E. coli and anaerobic bacteria strains with plasmids• Highly involved in cloning and sequencing of plasmids by using different techniques such as restriction, high-fidelity PCR, ligation, E. coli transformation, colony PCR and plasmid extraction• Responsible for the analysis/quality control of transformed clones by performing PCR, Western blotting and enzymatic assays• Responsible for maintaining Good Laboratory Practice and safety in the lab• Responsible for managing internal lab databases, such as chemicals stock• Student guidance About Syngip B.V.Syngip is a 3rd generation industrial biotech start-up created in 2014 in the Netherlands. It has developed a process to convert gaseous carbon sources such as CO2, CO, and industrial emissions such as syngas, into various valuable chemical compounds. Its major targets are light olefins, major petrochemical molecules, which include isobutene.Syngip is wholly-owned by Global Bioenergies S.A., located in Evry, France.About Global Bioenergies S.A.Global Bioenergies is one of the few companies worldwide, and the only one in Europe, that is developing a process to convert renewable resources into hydrocarbons through fermentation. The company initially focused its efforts on the production of isobutene, one of the most important petrochemical building blocks that can be converted into fuels, plastics, organic glass and elastomers. Global Bioenergies continues to improve the performance of its process, to operate its demo plant in Germany, and to prepare the first full-scale plant through a joint venture with Cristal Union, named IBN-One. Global Bioenergies is listed on Euronext Growth Paris (FR0011052257 – ALGBE).

Beek

• Making appointments for customers by phone and email• Receiving and forward customers• Answering questing by phone and email• Handling incoming mail• Making invoices• Arranging things last minute and switching fast between tasks

Department Of Human Movement Sciences & Human Biology

Senior Internship: The role of microRNA-150 in the regulation of skeletal muscle mitochondrial function Aim: Examined the effect of microRNA-150 silencing on mitochondrial oxidative capacity in C2C12 myotubesTechniques: Cell culture, transfection, qPCR, Western blotting, enzyme assays

Department Of Respiratory Medicine

Junior Internship: The impact of GSK-3β over-expression and activation on skeletal muscle oxidative metabolismAims: Examined the effect of GSK-3β over-expression in C2C12 myotubes on several markers of mitochondrial biogenesis and different OXPHOS complexes. Examined the effect of GSK-3β activation via LY294002 on several markers of mitochondrial biogenesis and different OXPHOS complexes in C2C12 myotubesTechniques: Cell culture, RNA isolation, cDNA synthesis, qPCR, Western blotting

Department Of Molecular Cell Biology

Bachelor Internship: Simultaneous detection of HPV and chromosomal aneusomy in uterine cervical intraepithelial neoplasia and adenocarcinoma in situ Aims: Optimisation of bicolour fluorescence in situ hybridisation (FISH) method to detect HPV and chromosomal aneusomy within a single FISH reaction. Evaluation of aneuploidy and physical status of HPV in patient tissue sectionsTechniques: FISH on HPV positive cell lines and patient tissue sections (CIN1, CIN2, CIN3, AIS lesions)

Master Of Science (M.Sc.), Biomedical Sciences

Master thesis: The role of microRNA-150 in the regulation of skeletal muscle mitochondrial function The master’s programme in Biomedical.

Bachelor Of Science (B.Sc.), Biomedische Wetenschappen (Biomedical Sciences)

Bachelor thesis: Simultaneous detection of HPV and chromosomal aneusomy in uterine cervical (pre)neoplasia The bachelor's programme.

Middelbare School, Gymnasium (Natuur & Gezondheid; Natuur & Techniek)