İpek Altinoglu-Bonnet Email & Phone Number

Paris, Fr

Île-De-France, France

My mission is to advance the frontiers of microscopy, fostering a culture of innovation that aligns with our organization's vision. My expertise in PALM, STORM, PAINT, TIRFM, SIM, Lattice-SIM and widefield microscopy techniques is instrumental in contributing diverse perspectives to our team, as we push the boundaries of scientific discovery from sample preparation to imaging and analysis.• Leading global product demonstrations and workshops to showcase innovative solutions•… Show more My mission is to advance the frontiers of microscopy, fostering a culture of innovation that aligns with our organization's vision. My expertise in PALM, STORM, PAINT, TIRFM, SIM, Lattice-SIM and widefield microscopy techniques is instrumental in contributing diverse perspectives to our team, as we push the boundaries of scientific discovery from sample preparation to imaging and analysis.• Leading global product demonstrations and workshops to showcase innovative solutions• Contributing to the company's growth by successfully closing high-impact projects and aligning with annual objectives.• Overseeing pre- and post-sales activities to ensure customer satisfaction.• Representing the company at international conferences, exhibitions, and seminars.• Collaborating on product development with expert understanding of technology and applications together with integrating customer feedback.• Managing advanced customer applications and providing tailored training and advance scientific support.• Creating marketing materials to effectively communicate product value.• Publishing application and technical notes to share industry insights.• Working closely with product development teams to refine and improve products. Show less

Paris Area, France

Article 1: New PALM-compatible integration vectors for use in the Gram-positive model organism Bacillus subtilis. ASM journals Microbiology Spectrum, Altinoglu I., and Carballido-Lopez R., 2023.https://doi.org/10.1128/spectrum.01619-24-Standardized PALM plasmids with dronPA and PAmCherry for fluorescent protein labelling in SMLM-Simultaneous dual color PALM in Zeiss ELYRA PS1-Fluorescent Reporter-Operator System (FROS) for DNA labelling in PALM-Live cell… Show more Article 1: New PALM-compatible integration vectors for use in the Gram-positive model organism Bacillus subtilis. ASM journals Microbiology Spectrum, Altinoglu I., and Carballido-Lopez R., 2023.https://doi.org/10.1128/spectrum.01619-24-Standardized PALM plasmids with dronPA and PAmCherry for fluorescent protein labelling in SMLM-Simultaneous dual color PALM in Zeiss ELYRA PS1-Fluorescent Reporter-Operator System (FROS) for DNA labelling in PALM-Live cell super-resolution microscopy-Structured Illumination microscopy for mono infected cell by SPP1 virus-Clustering with DBSCAN-Plasmid constructionsArticle 2: Systematical observation of viral infection in bacteria through super-resolution microscopy techniques such as SIM, Lattice-SIM and PALM-Organization of SPP1 infection in Bacillus subtilis in space and time.-Dual color, Trial color in 2D and 3D SIM imaging in Zeiss ELYRA PS1-Observation of bacterial chromosome in DAPI, viral DNA in LacI-mCherry (FROS) and viral factories (virus capsule etc.) in mCitrine.-Post image processing in FairSIM, ImageJ and Imaris.-SPT-PALM by using PAJF-549 Halotag conjugates in viral factories. Show less

Paris Area, France

Article 1: Single molecule super-resolution imaging of bacterial cell pole proteins with high-throughput quantitative analysis pipeline. Nature Scientific Reports. Altinoglu et al., 2019. https://doi.org/10.1038/s41598-019-43051-7-SMLM imaging of protein localization in human pathogens Vibrio cholerae.-Nikon NSTORM Mono-color, Dual-color in 2D and 3D imaging.-Designing of PALM compatible fluorophores such as PAmCherry, dronPA, mEoS2 and PA-GFP-Live cell SMLM imaging sample… Show more Article 1: Single molecule super-resolution imaging of bacterial cell pole proteins with high-throughput quantitative analysis pipeline. Nature Scientific Reports. Altinoglu et al., 2019. https://doi.org/10.1038/s41598-019-43051-7-SMLM imaging of protein localization in human pathogens Vibrio cholerae.-Nikon NSTORM Mono-color, Dual-color in 2D and 3D imaging.-Designing of PALM compatible fluorophores such as PAmCherry, dronPA, mEoS2 and PA-GFP-Live cell SMLM imaging sample preparation to image analysis.-Precise protein localization in cells by using coordinate tables and developed MatLab based scripts-Post processing in ThunderSTORM and NIS elements. Clusters analysis with SR-Tesseler. -Protein Co-localization in Icy SODA.Article 2: Analysis of HubP-dependent cell pole protein targeting in Vibrio cholerae uncovers novel motility regulators. Plos One. Altinoglu et al., 2022. https://doi.org/10.1371/journal.pgen.1009991-Discovery of 4 interactions partners of polar anchor protein in human pathogen Vibrio cholerae by using comparative proteomics and fluorescent microscopy imaging methods.-Subcellular localization and colocalization of polar proteins in Zeiss Observer.Z1-Tracking of swimming live cells in Leica DM6000-B.-Construction of fluorophores by using molecular biology techniques such as Gibson Assembly, enzyme digestion, ligation and PCR.PhD thesis, 26/10/18: Organization of bacterial cell pole Show less

Paris Area, France

Measurement of fluorescent labelled genes expression in real time with Nikon Ti2 microscopy equipped with PFS.-18h to 24h time-lapse microscopy-Single cell imaging in Escherichia coli-DNA repair proteins tagged with GFP and mCherry-Sample preparation for time-lapse imaging-Measurement of genes expression-Cell segmentation and image analysis

Doctor Of Philosophy

Doctor Of Philosophy (Phd)

Master'S Degree, Biotechnology

Molecular nano- and bio-photonics for telecommunications and biotechnologies

Bachelor Of Science (Bs), Engineer