Syed Irfan Bukhari Email & Phone Number

Cambridge, Massachusetts, Us

• Performed key MOA experiments in an early discovery biology program in oncology that helped identify the disease function of the target.• Performed pathway analysis to identify relevant biomarkers for patient population stratification• Performed IC50 studies for candidate molecules in different cancer cell lines to identify sensitive cancer cell lines• Contributed to the design, implementation, and optimization of all routine company assays (Western Blot, CRISPR, siRNA, ELISA, biochemical assays and cell-based in vitro and in vivo assays)• Managed CROs (FFS/FTE based) and relationships with vendors • Helped develop SOPs and implemented all lab guidelines and protocols for the whole company

Boston, Massachusetts, Us

• Established and lead the research program focused on the role of integrated stress signaling in regulating RNA methylation and ribosome changes in chemo-resistant breast cancer and leukemic cells• Generated knockdown and overexpression leukemic cell lines, Patient-derived 3D- Tumor spheroids to study chemo-resistance using proteomic analysis, and ribosome profiling• Contributed toward the federal and institutional grants for conducting the research.• Initiated and developed collaborations that bridged multiple institutes and disciplines. • Trained undergraduate summer students, rotating graduate students, and technicians in the lab.

Boston, Massachusetts, Us

Identified the mechanism of miRNA-mediated translation activation in quiescent & chemo-resistant cells.• Contributed to several collaborative projects identifying the non-canonical alternative translationmechanisms in chemo-resistant cells.• Studied the role of stress signaling in regulating RNA methylation and ribosome changes in chemoresistantbreast cancer and leukemic cells

Boston, Massachusetts, Us

• Conceptualized, independently designed, and executed a cutting-edge, multi-faceted research project that identified the mechanism of miRNA-mediated translation activation in quiescent and chemo-resistant cells• Identified and validated target genes and miRNAs that serve as biomarkers and drug targets using translational profiling, RNA-immunoprecipitation, and luciferase reporter assays• Established the role of PARN as a cap-binding protein involved in Poly (A)-independent mechanism of miRNP-mediated translation activation• Contributed to deciphering the role of p38 MAPK-MK2-TTP axis as a key regulator of AU-rich element (ARE) bearing mRNAs that promote chemoresistance

• Explored the underlying mechanism by which exogenous serotonin (5HT) regulates the trafficking and function of the serotonin transporter (SERT)• Investigated how 5HT levels influence the density of SERT molecules on the plasma membrane• Routinely maintained and handled rodents for blood collection and platelet isolation

• Studied the association between various polymorphisms/mutations in promoter elements of BRCA1/BRCA2 genes implicated in genomic instability and susceptibility to breast cancer using Sanger sequencing • Collaborated with colleagues to identify the role of mtDNA variants in providing susceptibility to Type 2 Diabetes in two North Indian populations using SSCP and RFLP assays

Doctor Of Philosophy - Phd, Cellular And Molecular Biology

Master'S Degree, Biotechnology