• Conducted IR band assignment of SAM vibrations as label-free probes and enzyme active site sensors.• Collaborated with computational labs for validation through DFT calculations and molecular dynamics.• Utilized SAM mimics and isotope labeling for accurate IR signal attribution to molecular vibrations.• Wrote a method manuscript (under review) for SAM synthesis and purification for infrared spectroscopic studies.• Produced novel IR spectra for labeled SAM derivatives… Show more • Conducted IR band assignment of SAM vibrations as label-free probes and enzyme active site sensors.• Collaborated with computational labs for validation through DFT calculations and molecular dynamics.• Utilized SAM mimics and isotope labeling for accurate IR signal attribution to molecular vibrations.• Wrote a method manuscript (under review) for SAM synthesis and purification for infrared spectroscopic studies.• Produced novel IR spectra for labeled SAM derivatives, advancing the project.• Enzymatically synthesized and purified isotopes (D3-methyl-SAM, 13C-methyl-SAM, and 15N-SAM).• Successfully separated SAM from impurities, obtaining pristine IR spectra.• Developed purification strategies using ion exchange and size-exclusion chromatography.• Optimized synthesis and purification of S-adenosyl-L-methionine (SAM) for IR studies.• Engineered Metk enzyme expression, ensuring optimal activity in SAM synthesis.• Identification and Structural Analysis of Highly-Selective, Potent NSD1 Lysine-methyltransferase Inhibitors using fluorescence coupled assay. Show less

• IR band assignment of SAM vibrations as label-free probes and sensors of enzyme active sites.Utilized SAM mimics and isotope labeling techniques to accurately attribute IR signals of SAM to their corresponding molecular vibrations. Efficiently collaborated with computational laboratories to validate band assignments via Density Functional Theory (DFT) calculations and molecular dynamics.• Optimized synthesis and purification of S-adenosyl-L-methionine (SAM) for infrared… Show more • IR band assignment of SAM vibrations as label-free probes and sensors of enzyme active sites.Utilized SAM mimics and isotope labeling techniques to accurately attribute IR signals of SAM to their corresponding molecular vibrations. Efficiently collaborated with computational laboratories to validate band assignments via Density Functional Theory (DFT) calculations and molecular dynamics.• Optimized synthesis and purification of S-adenosyl-L-methionine (SAM) for infrared spectroscopic studies. Engineered the expression and purification of Metk, an important enzyme in SAM synthesis, ensuring optimal enzymatic activity. Devised strategic employment of ion exchange and size-exclusion chromatography to overcome purification challenges, successfully separating SAM from excess sulfate salt and co-purifying counterions. Applied the optimized method to enzymatically synthesize and purify various isotopes of SAM, including D3-methyl-SAM, 13C-methyl-SAM, and 15N-SAM, leading to the acquisition of clean IR spectra. Produced the first-of-its-kind IR spectra for isotopically labeled SAM derivatives, thus propelling the project into its subsequent phase.• Mentoring, and training undergraduate students.Provided mentorship and training to more than 10 undergraduate students, resulting in their co-authorship in our publications and subsequent progression to graduate-level studies. Show less

• Facilitated exam collation for CHEM 1311 and CHEM 1312 (General Chemistry).• Coordinated laboratory activities for CHEM 3472 (Instrumental Analysis Lab).• Demonstrated procedures, answered queries, and assessed lab reports and presentations.• Offered constructive feedback to enhance student performance.• Mentored students through office hours and personalized communication.

• Led the study on a novel analytical approach based on hypochlorous scavenging to quantify antioxidant capacity in plant-based foods.• Employed HPLC and UV-vis instrumentation to detect and quantify the antioxidant capacities of green tea, black tea, and linden tea.• Significantly enhanced the precision of nutritional and biomedical analyses by providing analytical method that measures antioxidant content.

prepared and delivered lessons to a range of classes of different ages and abilities; graded, gave feedback on students' progress and development;Organizing Chemistry laboratory sessions and excursions to chemical industries;Liaised with other professionals, such as learning mentors, careers advisers, educational psychologists, and education welfare officers;Participated in and organized extracurricular activities, such as outings, social activities, and sporting events.

Evaluated quality throughout processes; Raw materials and API, Drug Products, Packaging components, Re-assay, Retains.Ensured efficacy, safety, quality, and compliance of drug products with the specification standards such as USP, BP, EP, and JP.Tested raw materials (Excipients, Capsules, Active ingredients) for purity, assay, and other physical tests on site before use; Tested packaging components such as bottles, blister pack components, cotton wool, and desiccants using a range… Show more Evaluated quality throughout processes; Raw materials and API, Drug Products, Packaging components, Re-assay, Retains.Ensured efficacy, safety, quality, and compliance of drug products with the specification standards such as USP, BP, EP, and JP.Tested raw materials (Excipients, Capsules, Active ingredients) for purity, assay, and other physical tests on site before use; Tested packaging components such as bottles, blister pack components, cotton wool, and desiccants using a range of techniques like Appearance test, Spectroscopy, Loss on drying etc.Tested finished products for the time of absorption into the bloodstream (dissolution test), Assay, content uniformity, hardness test, friability test, etc.Scheduled reassessment of released drugs to ensure continued quality using techniques such as HPLC, dissolution, reduced testing for re-assay, etc.Ensured all manufacturing equipment was cleaned by swabbing the equipment and testing using HPLC or by determining the concentration and comparing it with the Residue Limit (RL). Show less