Sarvani Indrakanti Email & Phone Number

Experienced professional in Molecular Biology (PCR, Real Time PCR Using Sybr Green and Novel Fret Techniques; Gel Electrophoresis; SDS - PAGE; Western Blotting, etc) and Bacteriology Research at CCMB with experience in handling various lab equipments.M.Sc. Biochemistry (Gold Medalist).

Listed skills include Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Western Blotting, Spectrophotometry, and 12 others.

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  Junior Research Fellow

  Centre For Cellular And Molecular Biology

  Feb 2015 - Present - Hyderabad Area, India

  Molecular Biology Techniques:• Extraction of Nucleic Acids• PCR, Real Time PCR Using Sybr Green and Novel Fret Techniques• Gel Electrophoresis• SDS - PAGE• Western Blotting • Quantification of DNA, RNA and Proteins by Automated Instrument (Nanodrop) and UV-Visible Spectrophotometer• Protein Quantification by Bradford Assay and Lowry’s Method.• Production of Taq DNA Polymerase and Pfu DNA Polymerase• Retrieval of Gene Sequences from NCBI and Sequence Analysis by Blast, Fasta and Genbank Bacteriology Techniques:• Sterilization Techniques• Isolation of Bacteria by Clinical Samples, Soil, Water Etc.• Media Preparation and Plating • Bacterial Staining and Microscopy• Antibiotic Sensitivity by Disc Diffusion Method