Isha Sharma Email and Phone Number
Isha Sharma personal email
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Pragmatic and goal-oriented postdoctoral research fellow studying pathogenesis of Diabetic Nephropathy. Prominently delineating mechanism which leads to chronic and acute kidney disorder in animal models. In addition I have always nurtured an aptitude for extracting simple and digestible information from complex scientific discoveries, which can give clear message to variety of audience. I must say I enjoy this skill to help common person and patients. Currently working towards designing small molecules that can potentially attenuate CKD progression. Looking for opportunity to combat renal or other healthcare disorder. In addition, as a scientist i have strong project management skills. Handled cross-functional team projects and communicated results to key opinion leaders in the field. Excellent writing and verbal communication skills ( gave 16+ international presentation and wrote 22 peer reviewed scientific articles). Ability to understand, integrate and effectively communicate scientific/medical findings to various key stakeholders. Integrated cross-functional project deliverables, plans, timelines, resources. I am highly motivated individual and have excelled in various cross-functional collaborations. Scientific Skills: Single cell RNA sequencing, Q-PCR, Western Blotting, Immunohistochemistry, Immunofluoresence, Mammalian cell culture, High throughput data analysis (single cell RNA sequencing), Mice model handling, Genotyping, Cell based assay, ELISA, Subtractive hybridization, Molecular cloning/sub-cloning, Protein Engineering, Recombinant protein production and purification, Polyclonal antibody production, Live cell imaging, Chromatin immunoprecipitation (ChIP), Gel-shift assay, Transfection, Antibacterial assay, Reporter assay, Tissue processing and sectioning both (Paraffin and cryosectioning), immunocytochemistry, MS office, Graph pad, PRISM, Photoshop, Image J. Soft SkillsTop Strengths: Honesty - Perseverance - Curiosity - Compassion - Fairness-Time management-Detail oriented-Ambidextrous- Tenacity.Keywords : Research - Project Management - Collaboration - Communication - Harmonizer - Scientific /medical Writer-Communication Management- Data analysis and evaluation.
Northwestern University - The Feinberg School Of Medicine
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Research Assistant ProfessorNorthwestern University - The Feinberg School Of MedicineChicago, Il, Us
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Research ScientistApt Therapeutics Jul 2024 - Present* Spearheading the project involved in determining the activity of recombinant proteins in attenuating ischemia-reperfusion injury in Large animal model. Cell-based assay, Single cell selection, Dot-blot, Generation of overexpression cell lines, Recombinant protein expression, Suspension cell culture, Generating recombinant protein for production and testing in animals. -
Research Assistant ProfessorNorthwestern University - The Feinberg School Of Medicine Jan 2015 - Jun 2024Chicago, Il, Us*My project at Feinberg School of Medicine revolves around pathogenesis of diabetic nephropathy. In the past six years, i got chance to delineate in detail molecular mechanism of acute and chronic kidney disease. * Methylation specific PCR indicated that our target enzyme (Myo-inositol oxygenase MIOX) is hypomethylated in hyperglycemic settings published in American journal of Pathology* Cisplatin is common anticancer drug, but is known for its nephrotoxicity. Using immunohistochemistry, florescence, Q-PCR, Methylation specific PCR, Immunoblotting, Cell culture, ELISA, Gel shift assay established that MIOX genetic deletion protect kidney against cisplatin induced injury (JASN, 2017)* Similarly, Tunicamycin ( inhibitor of Post translational modification) and long standing diabetes generate advance glycation end product showed accentuation of renal injury with MIOX overexpression ( AJP, 2018 & 2019). * Cell based assay and usage of various inhibitors suggested that MIOX overexpression exacerbates cell death in cisplatin induced AKI via accentuating ferroptosis and deletion attenuates renal injury ( JCI, 2019)* Used knockout and double knockout mice in diabetic and obese setting to establish the role of MIOX in diabetic nephropathy. * Present project: screening drug like small molecules which can potentially attenuate MIOX activity and contributes to attenuating progression of renal injury.* Skills developed and extensively used: Working with various disease mice models, Epigenetics, Cell culture, Generated overexpressing, knockout and mutated cell line, Gel shift assay, Subtractive hybridization, Histopathology, Immunohistochemistry, Cryosectioning, Immunofluorescence, Western blotting, ELISA, PCR, Q-PCR, In vitro transcription, Genotyping, Recombinant protein expression and purification, Cell based assay,Antibody generation, Random amplified cDNA ends, Co-culture ( cultured fibroblast and epithelial cell line).
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Volunteer ExperiencePet Cancer Foundation Jan 2021 - Jan 2022Converting complex scientific topics and making them simple and digestible for target audience has always attracted my attention. I am excited about this volunteering work, as i was surprised to know that pets ( dogs and cats) develop fatal cancer. I am working closely with group of scientific writers and generating material for Pet Cancer Foundation website. I sincerely hopes it helps the pets owner to better understand their disease progression, symptoms, outcomes and available treatments.
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Research AssociateCenter For Cellular And Molecular Biology Mar 2013 - Dec 2014*My project at CCMB, Hyderabad, India was "Production of transgenic mice secreting an echidna antibacterial protein in milk: Evaluation of its in-vivo activity in mouse model of mastitis". * The antibacterial activity of milk containing the EchAMP protein from transgenic mice against a host of bacterial pathogens was evaluated. Finally, the response of the mammary gland of the transgenic mice to experimentally induced mastitis was studied and published in BBA biomembranes-2019 and transgenic research -2019* Skills developed and extensively used: Vector engineering, Deletion construct, Promoter assay, Overexpressing cell lines, Cell culture, Primary cell culture, Working with mastitis induced transgenic mice models, Recombinant protein production and purification, Antibacterial assay, Genotyping, Southern, Northern, and Western blotting, PCR, Q-PCR.
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Graduate StudentDeemed University (Laboratory Of Endocrinology) Aug 2008 - Feb 2013Completed Ph.D. in Animal Biochemistry. My thesis revolved around elucidating the role of nuclear receptor peroxisome proliferators activated receptor-gamma (PPARg) in regulating steroidogenesis in ovary. * Using random amplified cDNA ends i was able to identify ovary specific novel isoform of PPARg which we named as PPARg1b (Gene, 2012)* We established serum free granulosa cell culture (DAE, 2011). * Elucidated the signaling mechanism regarding fertility regulation. Polyunsaturated fatty acid like conjugated linoleic acid (CLA) are administered in animals to treat negative energy balance. We established that CLA enhance ovulation via binding to PPARg and accentuating downstream signaling (Reproduction, 2012).
Frequently Asked Questions about Isha Sharma
What company does Isha Sharma work for?
Isha Sharma works for Northwestern University - The Feinberg School Of Medicine
What is Isha Sharma's role at the current company?
Isha Sharma's current role is Research Assistant Professor.
What is Isha Sharma's email address?
Isha Sharma's email address is an****@****ail.com
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