Isotta D'Arrigo

Isotta D'Arrigo Email and Phone Number

LCM Scientist @ Bavarian Nordic
Copenhagen, DK
Isotta D'Arrigo's Location
Copenhagen, Capital Region of Denmark, Denmark, Denmark
About Isotta D'Arrigo

I am a skilled and driven scientist with 5+ years of experience as microbiologist and molecular biologist. My research background extends from the analysis and optimization of microbial cell factories to the discovery of new antimicrobial therapies to treat bacterial infections. Overall, I am equipped with a varied toolbox of technical skills including microbial cultivation, DNA/RNA isolation, NGS, genome editing, phenotypic assays, and data analysis.As a scientist, my assets lie in designing protocols and implementing methods to answer biological questions combined with a strong passion to reach efficient workflows and high-quality results. Through training in big data analysis, science communication, and business development, I have also become an excellent communicator with capabilities to transform data into values. I enjoy being part of a team and reaching creative solutions with team members. I have been involved in interdisciplinary projects and have established rewarding collaborations, which have enriched my scientific background and increased research innovations. As a person, I am proactive and forward-looking. I positively accept new challenges as a way of professional development, and I am always open to learn new skills.

Isotta D'Arrigo's Current Company Details
Bavarian Nordic

Bavarian Nordic

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LCM Scientist
Copenhagen, DK
Employees:
1569
Isotta D'Arrigo Work Experience Details
  • Bavarian Nordic
    Lcm Scientist
    Bavarian Nordic
    Copenhagen, Dk
  • Glycom A/S
    Research Scientist
    Glycom A/S Dec 2019 - Present
    Hørsholm, Capital Region, Denmark
  • Dtu Bioengineering (Dtu Vet)
    Postdoctoral Researcher
    Dtu Bioengineering (Dtu Vet) Jul 2017 - Dec 2019
    Dtu Campus Lyngby
    - Elucidated the phenotype of antimicrobial resistance mechanisms in bacterial laboratory mutants and clinical isolates by performing antimicrobial susceptibility assays, growth analysis, and gene expression analysis (RT-qPCR, flow cytometry).- Isolated de novo antimicrobial resistant bacteria and developed growth assays to quantify mutation frequencies and determine bacterial fitness.- Introduced and performed novel genome engineering strategies (allelic exchange, MAGE, CRISPR/Cas9) for the construction of bacterial mutants.- Assessed the antimicrobial activity of new synthetic compounds as possible new antimicrobial drugs to use in infection disease. - Participated in the determination of the structure of bacterial membrane by permeability assays and membrane structure studies (negative surface charge, MALDI). - Established and maintained collaborations with interdisciplinary researchers inside and outside Denmark to develop strategies that reduce antimicrobial resistance evolution.- Planned bachelor and master students’ projects and trained students in the laboratory.- Contributed to the operational setting of a GMO2 laboratory by involvement in the establishment of equipment and regulations.
  • Evolva Sa
    Internship
    Evolva Sa Jan 2017 - Feb 2017
    Copenhagen Area, Denmark
    - Carried out the construction and screening of production yeast strains by applying yeast metabolic engineering strategies and performing HPLC analysis.
  • Dtu - The Novo Nordisk Foundation Center For Biosustainability
    Phd Fellow Eu Marie Curie
    Dtu - The Novo Nordisk Foundation Center For Biosustainability May 2013 - Apr 2016
    Kogle Alle 6, Dk - 2970 Hørsholm
    Thesis: “The transcriptional landscape of the production organism Pseudomonas putida”- Discovered novel putative RNA regulators (riboswitch, sRNA) and defined metabolic pathways associated with bacterial adaptation to stress conditions and performance in production scenarios by exploiting RNA-seq approaches and metabolic modeling.- Developed an in vivo expression assay to characterize the function of RNA regulators and elucidated the role of bacterial transporters by performing high-throughput analysis of bacterial growth.- Accomplished analysis of NGS data to generate transcriptomic maps, determine gene expression profiles, and identify novel genomic elements (ncRNAs, TSSs, ORFs, RBS).- Developed and optimized an in-vitro approach to discriminate RNA secondary structures.- Achieved successful collaborations to increase the impact of the research and participated in conferences and training courses to expand network and technical skills.
  • Novozymes
    Visiting Research Scientist
    Novozymes Mar 2015 - Apr 2015
    Bagsvard, Copenhagen
    - Characterized the function of a bacterial operon by construction of reporter and deletion strains to use in phenotypic analysis.
  • Københavns Universitet
    Master Thesis Project
    Københavns Universitet Feb 2012 - Aug 2012
    Copenhagen Center For Glycomics (Ccg), Faculty Of Health And Medical Sciences
    Thesis: “Glycopeptide microarrays for identification of type specific epitopes on viral envelope proteins”- Developed a microarray-based strategy to identify glyco-epitopes on viral proteins by performing peptide synthesis, MALDI characterization, and robotic printing of peptide libraries on microarray chip.- Discovered viral glycol-epitopes to use as biomarkers and vaccine development by sera screening on peptide microarray and identification of immunological peptides.
  • Università Degli Studi Di Firenze
    Bachelor Thesis Project
    Università Degli Studi Di Firenze Jun 2009 - Dec 2009
    Peptlab - Interdep. Laboratory Of Chemistry And Biology Of Peptides And Proteins
    Thesis: “Glycosylation of antigenic peptides and proteins by Cys-Ligation reaction” - Synthetized glycosylated antigenic probes (peptide and protein) to use for detection of autoantibodies as biomarkers of disease in multiple sclerosis.- Performed HPLC analysis to isolate peptide and used immunoassay (ELISA) to detect autoantibodies.

Isotta D'Arrigo Skills

Biotechnology Microbiology Molecular Biology Rna Biology Rna Isolation Rnaseq Polymerase Chain Reaction Plasmid Isolation Transcriptomics Expression Cloning Life Sciences Vector Cloning Data Analysis Biochemistry Bacterial Cell Culture Dna Extraction Bioinformatics Cell Biology Public Speaking Yeast Transformation Bacterial Transformation High Performance Liquid Chromatography Elisa Peptide Synthesis In Vitro Transcription Mass Spectrometry In Vitro Translation Microarray Biomarkers Organic Chemistry Radiolabeling Clc Main Workbench Vector Nti Project Management Interpersonal Skills Genome Editing Unix Organization Skills Screening Assay R Flow Cytometry Ngs Dna Sequencing Real Time Polymerase Chain Reaction Reverse Transcription Polymerase Chain Reaction Graphpad Prism Fluorescence Reporter Microtiter Plate

Isotta D'Arrigo Education Details

Frequently Asked Questions about Isotta D'Arrigo

What company does Isotta D'Arrigo work for?

Isotta D'Arrigo works for Bavarian Nordic

What is Isotta D'Arrigo's role at the current company?

Isotta D'Arrigo's current role is LCM Scientist.

What schools did Isotta D'Arrigo attend?

Isotta D'Arrigo attended Danmarks Tekniske Universitet, University Of Florence, University Of Florence.

What are some of Isotta D'Arrigo's interests?

Isotta D'Arrigo has interest in Traveling, Go Out With Friends And Family, Running, Play Tennis.

What skills is Isotta D'Arrigo known for?

Isotta D'Arrigo has skills like Biotechnology, Microbiology, Molecular Biology, Rna Biology, Rna Isolation, Rnaseq, Polymerase Chain Reaction, Plasmid Isolation, Transcriptomics, Expression Cloning, Life Sciences, Vector Cloning.

Who are Isotta D'Arrigo's colleagues?

Isotta D'Arrigo's colleagues are Jane Gerhard, Christopher Flokas, Christina Guttmann Lundegaard, Lijie (Lj) Chen, Michael Væver Jørgensen, Dalila Mendez Vega, Joakim Skou Thomsen.

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