I am a skilled and driven scientist with 5+ years of experience as microbiologist and molecular biologist. My research background extends from the analysis and optimization of microbial cell factories to the discovery of new antimicrobial therapies to treat bacterial infections. Overall, I am equipped with a varied toolbox of technical skills including microbial cultivation, DNA/RNA isolation, NGS, genome editing, phenotypic assays, and data analysis.As a scientist, my assets lie in designing protocols and implementing methods to answer biological questions combined with a strong passion to reach efficient workflows and high-quality results. Through training in big data analysis, science communication, and business development, I have also become an excellent communicator with capabilities to transform data into values. I enjoy being part of a team and reaching creative solutions with team members. I have been involved in interdisciplinary projects and have established rewarding collaborations, which have enriched my scientific background and increased research innovations. As a person, I am proactive and forward-looking. I positively accept new challenges as a way of professional development, and I am always open to learn new skills.
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Lcm ScientistBavarian NordicCopenhagen, Dk -
Research ScientistGlycom A/S Dec 2019 - PresentHørsholm, Capital Region, Denmark -
Postdoctoral ResearcherDtu Bioengineering (Dtu Vet) Jul 2017 - Dec 2019Dtu Campus Lyngby- Elucidated the phenotype of antimicrobial resistance mechanisms in bacterial laboratory mutants and clinical isolates by performing antimicrobial susceptibility assays, growth analysis, and gene expression analysis (RT-qPCR, flow cytometry).- Isolated de novo antimicrobial resistant bacteria and developed growth assays to quantify mutation frequencies and determine bacterial fitness.- Introduced and performed novel genome engineering strategies (allelic exchange, MAGE, CRISPR/Cas9) for the construction of bacterial mutants.- Assessed the antimicrobial activity of new synthetic compounds as possible new antimicrobial drugs to use in infection disease. - Participated in the determination of the structure of bacterial membrane by permeability assays and membrane structure studies (negative surface charge, MALDI). - Established and maintained collaborations with interdisciplinary researchers inside and outside Denmark to develop strategies that reduce antimicrobial resistance evolution.- Planned bachelor and master students’ projects and trained students in the laboratory.- Contributed to the operational setting of a GMO2 laboratory by involvement in the establishment of equipment and regulations.
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InternshipEvolva Sa Jan 2017 - Feb 2017Copenhagen Area, Denmark- Carried out the construction and screening of production yeast strains by applying yeast metabolic engineering strategies and performing HPLC analysis. -
Phd Fellow Eu Marie CurieDtu - The Novo Nordisk Foundation Center For Biosustainability May 2013 - Apr 2016Kogle Alle 6, Dk - 2970 HørsholmThesis: “The transcriptional landscape of the production organism Pseudomonas putida”- Discovered novel putative RNA regulators (riboswitch, sRNA) and defined metabolic pathways associated with bacterial adaptation to stress conditions and performance in production scenarios by exploiting RNA-seq approaches and metabolic modeling.- Developed an in vivo expression assay to characterize the function of RNA regulators and elucidated the role of bacterial transporters by performing high-throughput analysis of bacterial growth.- Accomplished analysis of NGS data to generate transcriptomic maps, determine gene expression profiles, and identify novel genomic elements (ncRNAs, TSSs, ORFs, RBS).- Developed and optimized an in-vitro approach to discriminate RNA secondary structures.- Achieved successful collaborations to increase the impact of the research and participated in conferences and training courses to expand network and technical skills. -
Visiting Research ScientistNovozymes Mar 2015 - Apr 2015Bagsvard, Copenhagen- Characterized the function of a bacterial operon by construction of reporter and deletion strains to use in phenotypic analysis. -
Master Thesis ProjectKøbenhavns Universitet Feb 2012 - Aug 2012Copenhagen Center For Glycomics (Ccg), Faculty Of Health And Medical SciencesThesis: “Glycopeptide microarrays for identification of type specific epitopes on viral envelope proteins”- Developed a microarray-based strategy to identify glyco-epitopes on viral proteins by performing peptide synthesis, MALDI characterization, and robotic printing of peptide libraries on microarray chip.- Discovered viral glycol-epitopes to use as biomarkers and vaccine development by sera screening on peptide microarray and identification of immunological peptides. -
Bachelor Thesis ProjectUniversità Degli Studi Di Firenze Jun 2009 - Dec 2009Peptlab - Interdep. Laboratory Of Chemistry And Biology Of Peptides And ProteinsThesis: “Glycosylation of antigenic peptides and proteins by Cys-Ligation reaction” - Synthetized glycosylated antigenic probes (peptide and protein) to use for detection of autoantibodies as biomarkers of disease in multiple sclerosis.- Performed HPLC analysis to isolate peptide and used immunoassay (ELISA) to detect autoantibodies.
Isotta D'Arrigo Skills
Isotta D'Arrigo Education Details
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Microbiology And Molecular Biology -
Faculty Of Pharmacy - Medicine And Surgery -
Faculty Of Agriculture - Pharmacy - Medicine And Surgery - Sciences Mfn
Frequently Asked Questions about Isotta D'Arrigo
What company does Isotta D'Arrigo work for?
Isotta D'Arrigo works for Bavarian Nordic
What is Isotta D'Arrigo's role at the current company?
Isotta D'Arrigo's current role is LCM Scientist.
What schools did Isotta D'Arrigo attend?
Isotta D'Arrigo attended Danmarks Tekniske Universitet, University Of Florence, University Of Florence.
What are some of Isotta D'Arrigo's interests?
Isotta D'Arrigo has interest in Traveling, Go Out With Friends And Family, Running, Play Tennis.
What skills is Isotta D'Arrigo known for?
Isotta D'Arrigo has skills like Biotechnology, Microbiology, Molecular Biology, Rna Biology, Rna Isolation, Rnaseq, Polymerase Chain Reaction, Plasmid Isolation, Transcriptomics, Expression Cloning, Life Sciences, Vector Cloning.
Who are Isotta D'Arrigo's colleagues?
Isotta D'Arrigo's colleagues are Jane Gerhard, Christopher Flokas, Christina Guttmann Lundegaard, Lijie (Lj) Chen, Michael Væver Jørgensen, Dalila Mendez Vega, Joakim Skou Thomsen.
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