Italo Tempera Email & Phone Number

Philadelphia, Pennsylvania, United States

Greater Philadelphia Area

Greater Philadelphia Area

Greater Philadelphia Area

•Conceiving and designing the project strategy•Performing and analyzing the experiments•Assisting other member of the lab in the project preparation•Overseeing undergraduate and rotation students project details•Corresponding with the PI to report progress, problems and other laboratory information

Greater Philadelphia Area

•Contributed to conceive and design the experimental strategy•Modified the Chromosome Conformation Capture (3C assay) in order to work for viral chromosome analysis later adopted by others members of the lab•Developed a new assay, Chromatin Loop Immunoprecipitation (ChLIp), to detect the involvement of specific chromatin binding factors in chromatin loop formation combining 3C and ChIp assays•Wrote the paper, published on PLoS Pathogens 2011, describing the methodology and outcome of these studies•Conceived, designed and wrote a successful NIH Pathway to Independence (PI) Award K99/R00 proposal

Rome Area, Italy

•Contributed to conceive and design the experimental strategy•Performed the experiments and analyzed the data•Presented the outcome of the research at both poster and podium presentation at national and international conferences•Assisted other members of the lab in preparation of the experiments•Contributed to write a paper published in 2007 on Infectious Agents and Cancer, describing the methodology and the outcome of these studies

Latina Area, Italy

•Developed the teaching material for undergraduate and graduate students•Presented to students modern biology techniques through discussing relevant scientific papers•Discussed potential applications of gene manipulation in agriculture and industry•Assigned to each student a topic relating to gene expression to present in front the class•Evaluated student performances by written tests

Rome Area, Italy

•Contributed to design the experiments•Analyzed by fluorescence microscopy the effect of PARP1 inhibitors on EBV positive cells•Monitored by flow cytometry the antiapoptotic effect of drug treatment in EBV induced cells•Analyzed the gene expression profile of viral lytic genes in response to drug treatment using RT-qPCR•Confirmed that PARP1 inhibitors affect viral lytic protein expression by Western blotting analysis•Evaluated the released of EBV viral particles in the media of cells treated with PARP1 inhibitors using Southern blotting analysis•Contributed to write a paper published in 2005 on Journal of Cellular Phisiology, describing the methodology and the outcome of these studiesc

Ph.D; Ph. D, Biochemistry

2003 B.Sc, Molecular Biology; Sciences