- Assisted in operations, plant molecular biology and construction of ECSIA's plant growth and water treatment system.- Aided in the successful testing of more than 200 varieties of vegetables and fruits.- Assisted in base line studies for water testing - Worked with teams to construct, break down and convert greenhouses using ECSIA's growing system.- Worked with founder on developing fish varieties used within the system.

Department of Medical and Molecular GeneticsNational Cell Repository for Alzheimer Disease

Manufactured and compounded more than 600 diagnostic reagents, calibrators, and controls with focus on Therapeutic Drug Monitoring Reagent systems and Drugs of Abuse Testing Reagent systems.▪ Accountable for the execution of validation protocols for new and existing diagnostic products developed and manufactured by Roche.▪ Key contributor to the successful completion of Roche’s Legacy Validation Project.▪ Key contributor to the successful completion of the Indianapolis to Mannheim transfer project.

Investigated molecular mechanisms of renal disease in the setting of HIV.▪ Conducted and monitored experiments utilizing technical and theoretical understanding of HIV.▪ Accountable for the accuracy, quality, and timeliness of experimental results; reported results on a weekly basis with lead investigator.▪ Contributed to the timely achievement of the overall project by analyzing experimental data, making recommendations, and acting independently to resolve scientific issues.▪ Organized a DNA and tissue repository comprising specimens from human research subjects.▪ Prepared manuscripts and presentations of experimental data.▪ Gained experience with kidney dissection and tissue slicing.▪ Kaufman,L., Yang, G., Hayashi, K., Ashby, J.R., Huang, L., Ross, M.J., Klotman, M.E., Klotman, P.E. (2007). The Homophilic Adhesion Molecule Sidekick-1 Contributes to Augmented Podocyte Aggregation in HIV-Associated Nephropathy. The Journal of the American Federation of Societies for Experimental Biology, 21, 1367-1375 doi: 10.1096/fj06-7191com

Investigated molecular mechanisms of HIV Associated Nephropathy.▪ Performed several molecular studies on the effect of HIV infection upon epithelial biology.▪ Became proficient in biochemical and molecular biology lab techniques such as real-time PCR, solid-phase extraction, ELISA, primary and transformed epithelial cell culture, T cell culture, murine modeling, in vivo measurement of renal function, paraffin embedding, nested PCR, immunohistochemistry, western analysis.