Antibody and protein engineering using phage display technology. Naive phage panning for reagent and potential therapeutic antibodies. Designed and panned affinity maturation libraries for affinity/activity improvement of phage or hybridoma derived antibodies.Humanization and optimization of potential therapeutic antibodies derived from hybridoma technology, including multiple projects moved into development.Transient protein expression and characterization of panels of antibodies by Biacore (kinetics), ELISA, Carterra (antibody binning and kinetics), and FACS. B cell cloning, expression, sequence analysis and characterization.Contributor to multiple patents and papers.

Antibody Engineering: Humanization and optimization of potential therapeutic antibodies derived from hybridoma technology.Stable cell line generation for monoclonal antibody production in NSO cells: cloning, transfection, subcloning, stability studies, and titer evaluation (Biacore C). Exploratory Technologies: protein expression, media optimization, assay automation (BiomekFX), and affinity determination (Biacore 3000)

- Research focusing on the mechanisms controlling blood cell formation, with a specific focus on the role of the signaling molecule STAT 3 and its regulation of the suppressor of cytokine signaling 3 and the transcription factor PU.1 in myeloid cell development.

Teaching Lab Coordinator for Microbiology and Immunology teaching labs, Managed student workers and lab teaching assistants