Jack Pu Email & Phone Number

•Gel and PCR purifications; subtarget, insert-vector ligations•Assemble/troubleshoot automated PCR robot; compile the work list from Blue Heron database and prep its necessary reagents•Set up subtarget PCR reactions utilizing the automated eight-probe robot; digest of subtargets.•Copy clone, nested, and fusion PCR; RCA (Rolling Circle Amplification)•Troubleshoot PCR problem w/ different techniques and additives•Relics; digest screen•Review DNA sequence with Consed

19310 North Creek Parkway, Suite 100, Bothell, Washington 98011

• Lab maintenance and cleaning; Washing and maintaining stocks of glassware• Reagent prep with aseptic techniques: buffers, fungal and bacteria plates, growth media, etc • Inventory control/ordering of all reagents and lab supplies• DNA cloning; PCR; restriction enzyme digestion for gene characterization and cloning; ligation • Gel and PCR purifications following SOP's• Fungal and bacteria culturing: streaking on plates and growing in liquid culture • DNA purification with mini, midi, and maxi prep• Fungal transfection using BIO-RAD He-system Ballistic Apparatus• Perform SDS-PAGE, coomassie blue staining and Western Blot Assay