Jacqueline Stein Email & Phone Number

West Sacramento, California, United States

- Work with MFG Associates and Quality Assurance to implement over 100+ new processes and procedures relating to buffer and media preparation- Manage troubleshooting of equipment while helping supervisors with process deviations, CAPAs, and change controls- Collaborate with Project Managers to onboard new chemicals into production, determine feasibility of potential products, as well as field responses to client queries- Assist in the product transfer from process development to manufacturing, creating and troubleshooting new product specifications through development runs- Participated in change management of company wide implementation of a facility expansion consisting of two 10000L and one 5000L stainless steel production tanks with integrated automation, as well the opening of an additional Animal Origin Facility for Cell Culture Media and Human and Bovine Sera production

West Sacramento, California, United States

- Worked in a cGMP cleanroom environment, obtaining in-depth knowledge of cGMP, aseptic technique, validation, process engineering, quality system regulations, and compliance of 21 CFR 210 & 211- Prepared and participated in the production of chemical solutions, batch sizes ranging from 2L to 10000L solutions, consisting of buffers, cell culture media, and cell culture sera- Coordinated the assembly and preparation of various production equipment, dispensing of buffer and media components, as well as performance of basic laboratory tasks, such as monitoring pH, conductivity, test product samples, sterile filtration, and filter integrity testing- Completed and maintained batch record and logbook documentation in accordance with GDP

- Lab assistant to Dr. Kimberly Mulligan, investigating the neurodevelopmental effects of untested environmental chemicals on Drosophila melanogaster (fruit flies) that hold a common single-gene mutation that increases the risk of Autism in humans.- Planned and implemented protocols relating to SDS-PAGE and Western Blotting to enhance data collection used in graduate students’ research- Performed various experiments using immunohistochemistry and confocal microscopy, collecting data on neural stem cells of DrosophilaNguyen, U., Tinsley, B., Sen, Y., Stein, J., Palacios, Y., Ceballos, A., Welch, C., Nzenkue, K., Penn, A., Murphy, L., Leodones, K., Casiquin, J., Ivory, I., Ghenta, K., Danziger, K., Widman, E., Newman, J., Triplehorn, M., Hindi, Z., & Mulligan, K. (2021). Exposure to bisphenol A differentially impacts neurodevelopment and behavior in drosophila melanogaster from distinct genetic backgrounds. NeuroToxicology, 82, 146–157. https://doi.org/10.1016/j.neuro.2020.12.007