Created and modified lesson plans to meet the needs of all studentsAttended various biology team meetings and professional development meetingsCoached JV Girls soccer / Undefeated district championsReceived influential educator award from current studentValid CDL class B holder

Created and modified lesson plans to meet the needs of all studentsAttended various biology team meetings and professional development meetingsAttended Avid Summer Institute – San Antonio 2022Attended Challenge Day emotional support workshop at AHS – Spring 2023Received influential educator awards from former and current studentsCoached 1 season of girls and boys cross country, girls volleyball, and 2 seasons of girls soccer

Utilize liposomal drug delivery method to study and reduce the symptoms of preterm labor.Perform ELISAs and activity assays to assess for levels of PGE and Cox1/Cox2 enzyme in mouse and human uterine tissuesPerform immunofluorescence (IF) on frozen and paraffin embedded tissues to compare differences in protein expression in two-dimensional (2D) and three-dimensional (3D) uterine tissues

Teach 6th grade neuroscience and 8th grade biotechnology classesPrepare curricula, laboratory and classroom activities, and grade assignments

* Design and perform three-dimensional (3D) cell culture experiments based on a novel magnetic tissue engineering system designed to generate more en vivo like results* Responsible for optimizing 3D cell culture experiments from single well to multi-well high throughput screening for various cell types and compounds of interest* Conduct experiments as a contract research organization (CRO) for several laboratories and pharmaceutical companies * Process images using a custom Python program developed in house by Nano 3D* Meet current and prospective clients to maintain and secure business* Represent the company at vendor shows and conferences* Assist with revising protocols, product user manuals, and company flyers* Train and provide technical support and assistance for product end users* Perform viability assays (MTT, cell titer glo), migration assays, and immunohistochemistry (IHC)* Supervise seasonal research personnel * Responsible for maintaining tissue culture, purchasing, records, and miscellaneous lab duties

* Studied the role of beta-blockers, beta agonists, and PKA inhibitors in the cAMP/PKA signaling pathway for the regulation of mucous production in Normal Human Bronchial Epithelial (NHBE) cells. * Performed IHC and ELISAs to assess for the level of MUC5AC qualitatively and quantitatively expressed in NHBE cells. * Investigated the role Rab GTPases in intracellular trafficking of Beta 2 adrenergic receptors expressed in transfected HEK 293 cells by means of siRNA knock down or plasmid DNA transfection to alter endogenous protein expression.* Performed flow cytometry, ELISAs, radio ligand binding assays, and fluorescence microscopy to study the kinetics of Beta 2 adrenergic receptors * Collaborated with Nano 3D Biosciences, Inc. to investigate the role of magnetically levitated (3D) cell cultures against traditional (2D) cell cultures to assess for differences in extracellular matrix (ECM) expression upon stimulation with pro-fibrotic drug treatments. * Followed various specimen-processing protocols for clinical samples, data entry into CliniTaraq specimen tracking system, record keeping, and shipping, for a multi-site study (CTOTC-03) sponsored by NIAID and NHLBI.* Developed western blots for various proteins of interest.* Purified plasmid DNA from E. Coli * Maintained mammalian primary and cell line cultures for the duration of all studies* Utilized de-convolution fluorescent microscopy to create 3D stacks of 2D images * Processed images in MetaMorph and PhotoShop software * Kept records of all experiments and clinical studies

* Prepared custom antibody conjugates (HRP, FITC, and Alkaline Phosphatase) for use in ELISA kits and for immunohistochemistry purposes* Purified antibodies from animal serum and plasma with affinity chromatography* Performed ELISAS and Immuno-electrophoresis for quality control purposes* Followed general production management protocols standard operating procedures* Spun down samples, obtained optical density readings, calibrated pH meters, and kept records* Prepared buffers and solutions