Jacob Mehlhoff Email & Phone Number

Winston-Salem, Nc, Us

Remote

• Analyzed variant specific information and case level data to determine pathogenicity of detected variants using published scientific literature, internal data, and publicly available databases• Inspected clinical and genetic information across targeted sequencing, next generation sequencing, whole exome sequencing, and whole genome sequencing platforms with particular focus in genes associated with neurodevelopmental, cardiological, and rare disorders• Conducted SQL-based data pulls and developed visuals to improve company performance via data driven decision making as part of the Assay Care team• Supported the training program by cross-checking workflows and providing feedback to trainees

Ostermeier Lab, Croft Hall, Baltimore, Maryland, United States

• Constructed comprehensive libraries of over 60,000 total single-codon substitutions across four antibiotic resistance proteins and measured collateral fitness effects of these variants using deep mutational scanning in the absence of antibiotic• Broadened the scope of the laboratory and decreased turnaround time by writing Python scripts and detailed instructions for the analysis of deep sequencing and RNA-seq data. Computational work had previously been outsourced to a bioinformatics lab at the Walter & Eliza Hall Institute of Medical Research• Measured changes in expression of 4,700 genes across selected mutations using RNA-seq• Developed a protocol for fractionation of bacterial cells to investigate mechanisms of collateral fitness effects• Mentored four graduate students and three undergraduate students. Three of these students are currently in PhD programs while another is beginning an MD-PhD

Homewood Campus, Baltimore, Maryland, United States

• Provided guidance during weekly two-hour meetings with teams and additional office hours as students worked on their semester design project• Presided over transition to remote access of ASPEN software and individually met with students to troubleshoot issues during start of COVID-19 pandemic• Received a Whiting School of Engineering Award for Outstanding Teaching Assistant

Baltimore, Maryland, United States

• Invited by Chief Science Officer of Dipole Materials, Inc. to operate Elmarco electrospinning machine to create N85-N95 grade nanofiber filter material during the COVID-19 pandemic. Material produced was used in creating 150,000-250,000 masks distributed throughout Maryland• Performed quality control tests to ensure efficacy and consistency of nanofiber filter material

Homewood Campus, Baltimore, Maryland, United States

• Led a weekly three-hour homework session addressing questions as students worked through problem sets• Generated solutions for problem sets and graded both problem sets and exams• Invited as a guest lecturer to teach a lecture on Monte Carlo methods in October 2018

Murphy Lab, Engineering Hall, Madison, Wisconsin, United States

• Characterized the recombinant protein human cystatinC and its formation of oligomers• Investigated the mechanism of fibril formation for wild-type and mutants of cystatinC• Studied the regulation of beta-amyloid by cystatinC in relation to Alzheimer’s disease

Weidner Lab, Columbia, South Carolina, United States

• Tested the effects of varying process conditions in order to optimize the hybrid sulfur system• Modified the experimental setup in order to ease collection of data• Analyzed the efficiency of various fuel cells and electrolyzers

Chemical & Biomolecular Engineering, Protein Engineering

Activities and Societies: Graduate Student Liaison Committee (GSLC) Social Chair ; American Institute of Chemical Engineers (AIChE).

Bachelor'S Degree, Chemical & Biological Engineering