Jacob Mehlhoff Email & Phone Number

Remote

• Analyzed variant specific information and case level data to determine pathogenicity of detected variants using published scientific literature, internal data, and publicly available databases
• Inspected clinical and genetic information across targeted sequencing, next generation sequencing, whole exome sequencing, and whole genome sequencing platforms with particular focus in genes associated with.
• Conducted SQL-based data pulls and developed visuals to improve company performance via data driven decision making as part of the Assay Care team
• Supported the training program by cross-checking workflows and providing feedback to trainees

Ostermeier Lab, Croft Hall, Baltimore, Maryland, United States

• Constructed comprehensive libraries of over 60,000 total single-codon substitutions across four antibiotic resistance proteins and measured collateral fitness effects of these variants using deep mutational scanning in.
• Broadened the scope of the laboratory and decreased turnaround time by writing Python scripts and detailed instructions for the analysis of deep sequencing and RNA-seq data. Computational work had previously been.
• Measured changes in expression of 4,700 genes across selected mutations using RNA-seq
• Developed a protocol for fractionation of bacterial cells to investigate mechanisms of collateral fitness effects
• Mentored four graduate students and three undergraduate students. Three of these students are currently in PhD programs while another is beginning an MD-PhD

Homewood Campus, Baltimore, Maryland, United States

• Provided guidance during weekly two-hour meetings with teams and additional office hours as students worked on their semester design project
• Presided over transition to remote access of ASPEN software and individually met with students to troubleshoot issues during start of COVID-19 pandemic
• Received a Whiting School of Engineering Award for Outstanding Teaching Assistant

Baltimore, Maryland, United States

• Invited by Chief Science Officer of Dipole Materials, Inc. to operate Elmarco electrospinning machine to create N85-N95 grade nanofiber filter material during the COVID-19 pandemic. Material produced was used in.
• Performed quality control tests to ensure efficacy and consistency of nanofiber filter material

Homewood Campus, Baltimore, Maryland, United States

• Led a weekly three-hour homework session addressing questions as students worked through problem sets
• Generated solutions for problem sets and graded both problem sets and exams
• Invited as a guest lecturer to teach a lecture on Monte Carlo methods in October 2018

Murphy Lab, Engineering Hall, Madison, Wisconsin, United States

• Characterized the recombinant protein human cystatinC and its formation of oligomers
• Investigated the mechanism of fibril formation for wild-type and mutants of cystatinC
• Studied the regulation of beta-amyloid by cystatinC in relation to Alzheimer’s disease

Weidner Lab, Columbia, South Carolina, United States

• Tested the effects of varying process conditions in order to optimize the hybrid sulfur system
• Modified the experimental setup in order to ease collection of data
• Analyzed the efficiency of various fuel cells and electrolyzers

Chemical & Biomolecular Engineering, Protein Engineering

Activities and Societies: Graduate Student Liaison Committee (GSLC) Social Chair ; American Institute of Chemical Engineers (AIChE).

Bachelor'S Degree, Chemical & Biological Engineering