Jacob Weber Email & Phone Number

New York, Ny, Us

Sydney, Nsw, Au

Hiked the Pacific Crest Trail, a 2650 mile (4265 km) backpacking journey from Mexico to Canada through the mountains of California, Oregon, and Washington

Tarrytown, New York, Us

Process Development o Developed 5 commercial bioreactor processes using Quality by Design and Design of Experiment principles to optimize production of monoclonal antibodies and ensure robust quality attributes.o Led internal technology transfer of 2 commercial bioreactor processes to GMP manufacturing environment (2000L scale).o Design and execute pilot-scale (up to 500L) experiments in single-use and stainless-steel bioreactors to characterize new processes, cell lines, and cell culture media.o Build multifactor statistical models of bioreactor processes to improve understanding of upstream processes and potential scale effects. o Perform preventative maintenance and routine troubleshooting of bioreactor equipment. Process Automation & Operational Technologyo Design robust automated processes for bench-top and pilot-scale bioreactors using PLC programming, Rockwell FactoryTalk, Induction Automation Ignition, AVEVA PI, and Optimal synTQ.o Integrate new sensors to PLCs, develop control logic to automate new processes, and connect multiple PLCs to site DCS (FactoryTalk View SE).o Evaluate and develop new automation technologies including MAST automated sampling system to reduce operator workload, increase process consistency, and enable advanced process control. o As team AVEVA PI Administrator, onboard and connect new equipment and processes to PI historian, develop new analyses, visualization tools, and alarms for operators to monitor automated processes, and maintain and upgrade PI system. Laboratory Sustainabilityo Developed sustainability initiatives to increase lab consumables plastics recycling, resulting in 11,800 kg recycled and an estimated 544 metric tons CO2e reduced compared to incineration from 2020 to 2023.o Led department to achieve first My Green Lab Certification (Platinum level) for Regeneron, and shared strategie with another 14 labs working towards Certification.

Tarrytown, New York, Us

Tarrytown, New York, Us

San Francisco, Ca, Us

Participated in a twelve-week cohort-based program designed to cultivate collaboration and connectivity among climate professionals, startup founders, scientists, engineers, designers, investors, and subject-matter experts.

Cambridge, Ma, Us

Performed technology diligence and analysis on precision fermentation, food technology, and climate technology start ups for venture capital firms and institutional investors.

Set up and operation of 7L Applikon bioreactor for fermentation of engineered yeast (Pichia pastoris) strains to produce mutant alpha-galactosidase for enzyme replacement therapy research.Separation and purification of enzyme using tangential flow filtration and diafiltration.Perform enzyme assays and create substrate saturation curves to determine enzyme kinetics.

Ludwigshafen, De

Overhauled site process information management system (PIMS) to streamline data management and visualization across research, pilot, and manufacturing scales.Collaborated with fermentation scientists to refine MS Excel dashboards for offline data upload and interfaced with engineers and operators to integrate the process into their workflow.Conducted hands-on training sessions and developed troubleshooting documentation for new software.Facilitated development of global standards for transfer of updated data management to multiple BASF sites.

Ludwigshafen, De

Engineering and fermentation of industrial host strain for enzyme production.Performed and troubleshot bacterial transformations, PCR experiments, DNA gel electrophoresis, media optimization studies, and enzyme assays.Set up and operated lab scale bioreactors from one to five-liter reactor volumes.Collaborated with international scientists and engineers on project updates and timeline deliverables.Analyzed data and created spreadsheets, lab reports, and presentations.

Synthesize polypeptides using Fmoc solid-phase peptide synthesis. Analyze secondary structure with circular dichroism. Perform microrheology to study peptide self-assembly kinetics.Utilize ImageJ and Matlab to calculate material properties of resulting peptide hydrogel. Selected Publication: S. C. O’Neill, A. D. Kanthe, J. A. Weber and R. S. Tu, “ ‘Reverse’ Hofmeister effects on the sol–gel transition rates for an α-helical peptide–PEG bioconjugate,” Phys. Chem. Chem. Phys., 2018, 20, 20287, DOI: 10.1039/C8CP03316A

Franklin Lakes, New Jersey, Us

Performed mechanical testing including tension, compression, and torsion measurements on BD products using Instron Testing machines.Collected and analyzed data with Microsoft Excel and Minitab, and reported results to R&D management and peers with spreadsheets, presentations, and technical reports.Collaborated with lab technicians, engineers, and marketing managers to complete project deliverables within set timelines.Continued ongoing shelf-life experiments for new materials design of blood collection tubes.Partook in validations testing of developing laboratory procedures.

Trained and assisted structural design consultants in Autodesk Inventor software.Organized and facilitated drawing and spreadsheetuse for structural design consultants.