• Develop analytical methods using qPCR, immunoprecipitation, and other PCR-based strategies including digital PCR and sequencing for RNAi therapeutics• Design specific RT-qPCR primer and probe sets for target sequences • Develop diverse RNA/DNA extraction procedures for cells, tissues, and biofluids • Execute high level data analysis for document creation and internal presentation

• Executed in-vivo studies and developed molecular analyses of biological tissues to advance novel RNAi treatments for triplet repeat disorders• Analyzed tissues through RNA/DNA extractions for RT-qPCR, qPCR, and Western blot • Prepared frozen tissue samples for downstream applications • Managed and organized experimental drug and tissue databases

• Study Machado-Joseph disease to discover how the mutant gene kills neurons• Investigate the effects of upregulating antioxidants, transcription factors, and heat shock proteins on dysfunctional MJD protein aggregates to cause disease rescue• Perform mating crosses on Drosophila and analyze protein expression through Western blotting and frozen sectioning• Assist in laboratory duties including organizing flies, making fly food, and cleaning vials • Present findings through a… Show more • Study Machado-Joseph disease to discover how the mutant gene kills neurons• Investigate the effects of upregulating antioxidants, transcription factors, and heat shock proteins on dysfunctional MJD protein aggregates to cause disease rescue• Perform mating crosses on Drosophila and analyze protein expression through Western blotting and frozen sectioning• Assist in laboratory duties including organizing flies, making fly food, and cleaning vials • Present findings through a scientific poster in research symposiums Show less

• Conducted literature search of experimental and theoretical data regarding mechanisms of drug delivery and distribution of recombinant human Arylsulfatase A (rhASA) from the CSF to the white matter of the brain as well as its intracellular trafficking pathways.• Compiled total knowledge and historic data of nonclinical and clinical trial data for rhASA by meeting with various functional area leads• Organized and updated extensive Metachromatic Leukodystrophy bibliography and visual… Show more • Conducted literature search of experimental and theoretical data regarding mechanisms of drug delivery and distribution of recombinant human Arylsulfatase A (rhASA) from the CSF to the white matter of the brain as well as its intracellular trafficking pathways.• Compiled total knowledge and historic data of nonclinical and clinical trial data for rhASA by meeting with various functional area leads• Organized and updated extensive Metachromatic Leukodystrophy bibliography and visual summary slide deck• Presented findings in various functional group meetings Show less