Jada M. Email & Phone Number

Duarte, Ca, Us

Responsible for immune monitoring for Clinical Vaccines DevelopmentIsolation and cryopreservation of PBMCs and serum from immunocompromised clinical trial patients in COVID vaccine clinical studiesPerformed human serum heat-inactivation for immunoassaysTransfection of HEK293-17 cell line to produce COVID pseudo-virus variants.Neutralization assays on serum collected from clinical trial patients to assess immunity over time against COVID variants.In vitro analysis of serum from clinical trial patients and lab animal (mice) serum using IgG ELISASupported Regulatory Affairs in developing new and updating older SOPs, relevant to laboratory team operations

Pasadena, California, Us

Pasadena, California, Us

Routinely managed Flow Cytometer Setup and Tracking analysis as well as troubleshooting emergent issuesSOP development for troubleshooting FACS machine issues and using Benchling software for storage of samplesHigh-throughput processing of donor blood (leukopaks) to derive immune cells for drug testing and development Carried out T-cell and NK cell isolation from PBMCs for binding and functional assaysIsolation of monocytes from PMBCs to different into macrophageResponsible for expanding and maintaining up to ten different cancer cell lines at a timeIFN-gamma, IL-4, and TNF- alpha cytokine detection in cell culture supernatant using ELISA and Meso-Scale Discovery immunoassaysData analysis of binding assays to evaluate and compare binding affinity of various drug targets using GraphPad Prism 8Transferred paper and spreadsheet records into a cloud-based (Benchling) method for reliable record-keeping of stored samplesCryopreservation of PBMCS and various cancer cell lines and Cell Bank inventory management using the Benchling softwarePeer training of proper handling of human blood products and immune cell isolation as well as immunoassays

Los Angeles, Ca, Us

Biobank and Translational Research CoreFicoll isolation and cryopreservation of peripheral blood mononuclear cells (PBMCs)Inventory maintenance and management of stored tissue samples using LabVantage softwareLaboratory processing and storing of serum, plasma, and PBMCs from blood and various organ tumor tissues collection from cancer patient volunteers in collaboration with various clinical research groupsInformed consent verification for clinical research in accordance with good clinical practice (GCP)Accessioning and preparation of samples for external laboratory shipment

Claremont, Ca, Us

Working to determine a possible vehicle to deliver CRISPR-Cas9 across the blood brain barrier, to the brain for treatment of neurodegenerative disorders. Current methods of delivery are invasive, so through my research, I am looking to find a less invasive method of delivery. Through this research, I am gaining experience in writing and troubleshooting protocols, project planning, public speaking though giving presentations, aseptic techniques, mammalian cell culture, Size Exclusion Column (SEC) using NanoTracking Analysis (NTA), confocal microscopy, Ussing Chamber system, and ELISA.Key accomplishments:BSL1 and BSL2 trainingAseptic techniquesMaintaining mammalian cell cultureProject developmentTroubleshooting and protocol writing/modificationsDynamic light scatteringSize exclusion chromatographyNano-tracking analysisWestern BlotELISAH&E StainingTraining studentsAutoclave/sterilizing materials

Claremont, Ca, Us

Assisted a PhD candidate with her project in understanding the role of young and old erythrocyte-derived exosomes in aging, and their effect on the integrity of the blood-brain barrier (BBB). Exosomes were isolated from the whole blood of young and old aged mice through using a density-based centrifugation, then purified using size exclusion chromatography. Afterwards, their presence was validated using dynamic light scattering, Nanotracking Analysis (NTA), western blot and ELISA. Once the presence of exosomes were validated, other studies were done to study their effects on the blood-brain barrier to understand their effects on aging. Through this research, I was also able to gain experience in mammalian cell culture and sterilization techniques, along with project planning and writing protocols. From understanding the effects of exosomes isolated from young and old red blood cells, we can better understand how neurodegenerative disorders may occur to identify better treatments.Key accomplishments:• BSL1 and BSL2 training• Aseptic techniques• Maintaining mammalian cell culture• Project development• Troubleshooting and protocol writing/modifications• dynamic light scattering• size exclusion chromatography• dynamic light scattering• Nano-tracking analysis• Western Blot• ELISA

New Orleans, Louisiana, Us

-Manage the residence hall -Run monthly floor meetings and conduct room checks to ensure students are abiding by the rules and regulations-Work at the front desk of the residence hall approximately 20 hours a week and perform duties such as answering questions and concerns residents may have.-Enforce the university and residence hall rules and regulations to ensure that hall is a safe and orderly environment.-Maintain bulletin boards and fliers.-Counsel students on their personal and academic issues.-Plan and give educational and fun hall programs for residents two or three times a month.

Rochester, Ny, Us

Assisted a PhD candidate in understanding the effects of statin drugs in Long QT Syndrome Type 2. Various statin drugs were tested on different mutants of the hERG channel in HEK cells. My role included making various mutants of the hERG channel through subcloning, then transfected the mutants into the cells. Once transfected, various statin drugs were tested on the mutant in vitro. Afterwards, the expression of the hERG protein were validated using Western Blot to examine if the drug upregulated or downregulated the hERG protein.Key accomplishmentsPerformed sub-cloning through PCR and ligation.Grew mammalian and bacterial cell culturesPerformed mini and midi-prepTransfected cellsPerformed Western Blots and DNA Electrophoresis

Master Of Science - Ms, Applied Life Sciences

Bachelor Of Arts - Ba, Biology, General

High School Diploma