Jaee Shah Email & Phone Number

San Carlos, Ca, Us

San Carlos, California, United States

Antibody Inventory and QC

San Carlos, California, United States

Antibody QC and Inventory

South San Francisco, California, United States

Antibody QC and Inventory

San Antonio, Texas Area

Characterisation of tick and insect chemosensory receptors by various analytical methods.Relevant techniques used: SDS-PAGE, Native PAGE, In-gel trypsin digestion, UV-Visible spectroscopy, Protein purification by preparative Size Exclusion Chromatography, Dot blot, Imaging mass spectrometry, Fluorescent binding assay, proteomic analysis by MASCOT and MaxQuant.

San Antonio, Texas Area

Tutoring undergraduate students for General chemistry, Organic Chemistry and Genetics at Thomas Rivera centre . Managing the Chemistry summer camp by tutoring the enrolled students and grading their quizzes.

Bothell, Washington

Development of low artifact peptide mapping platform method for analysis of antibody drug conjugates and monoclonal antibodies.Skills developed: Peptide mapping, analysis of post translational modifications by LC-MS/MS, reduced mass analysis by LC-MS, experimental data recording in electronic lab notebook and presentation of work in team meeting.Instruments handled: Mass Spectrometry (Thermo Scientific-Q Exactive and Q-Exactive HFX, Bruker maxIS II ETD), UPLC (Waters).Softwares used: Protein Metrics, Thermo Xcalibur, GPMAW.

Pune

My key performance area included physiochemical characterization of Biosimilars (Monoclonal antibodies, fusion proteins and small molecule) by various analytical methods (HPLC and LC-MS). -Support in analytical method development of chromatographic separation-based techniques like Reverse Phase, Size Exclusion, Cation Exchange and Affinity chromatography for assessing the quality and purity of biosimilars. Routine analysis with existing protocol based chromatographic methods. -Qualification of Ion exchange and O glycan methods by ICH guidelines.-Characterization of biosimilars by Q-Triple TOF Mass Spectrometry by studying the molecule characteristics like intact mass, reduced mass, amino acid sequence, N and C terminal heterogeneity, disulphide linkages, N and O-linked glycans and various post translational modifications. -Biosimilarity exercise to compare Lupin Biosimilar with Reference Medicinal Product for the purpose of PMDA submission. -Forced degradation studies and Stability studies (Real time and accelerated) of Drug Substance and Drug Product. -Recording experimental results in Laboratory note book and test data sheets, preparation of analytical documents like Method Development Report, Method of Analysis, Standard Testing Procedure and Test Data Sheets. Support in the preparation of study reports for the purpose of regulatory submission. -Additional responsibilities: Maintenance of reagent inventory, inventory of samples received and analyzed. Training of new employees and interns on various analytical methods and SOPs.Instruments handled: Mass Spectrometry (AbSciex-4600 and 5600), UPLC (Waters), HPLC (Agilent-1260 infinity).Softwares used: Chemstation, Empower, Analyst, Biopharma View, Peak view, Sim glycan, Protein Metrics.

Pune Area, India

Physiochemical characterization of monoclonal antibodies (MAbs) produced by Mammalian cell line – CHO by various HPLC-based analytical techniques. Summary: -Analysis of in-process samples from Upstream and Clone Development team. Estimation of protein titre of Shake flasks and Fermenter samples. Technique used-Affinity chromatography (Recombinant Protein A). -Analysis of in-process samples from Downstream teamRelative estimation of charged variants and aggregates at every step of downstream operations. Technique used- Ion Exchange chromatography (Strong Cation Exchange) and Size Exclusion chromatography. -Analysis of IgG purity under reducing and non-reducing conditions by Capillary electrophoresis (CE-SDS). -Stability studies and Forced degradation studies of Drug substance using various stress conditions (pH, temperature and oxidizing agent). Relevant skills learnt- Buffer preparation, Operation, handling and troubleshooting of HPLC systems, Data analysis and reporting, SDS-PAGE. Instruments handled: HPLC (Agilent-1260 infinity), UPLC (Waters-I and H class), Shimadzu (LC 2010), CE (Beckman Coulter).Softwares used: EZ Chrome elite, Openlab CDS, Chemstation, LC solutions, Empower.

Bangalore

Project topic-Model based analysis of growth in Actinomycetes. Project summary- Media optimization for the production of Macrolide immunosuppressants by microbial fermentation and model-based analysis using the Cell Net Analyser software.Relevant skills learnt- Media preparation,measurement of biomass, harvesting of cells for the extraction of product. Analysis of fermented broth for the consumption of reducing sugar, glycerol and amino acids (using HPLC system), determination of total ammonical nitrogen and phosphorous (using continuous flow analyser), determination of total protein content by Kjeldahl method and raw material analysis. Calculation of carbon flux in the major carbon catabolic pathways of Streptomyces sp.using MATLAB based Cell Net Analyzer. Study of batching of small scale fermenters (10 l fermenter).

Navi Mumbai

The project was based on :• Correlation study of Apolipoprotein E (ApoE) polymorphism with Coronary Artery Disease (CAD) risk. In this study, the role and frequency of occurrence of six genotypes of Apo e in CAD population in Western India was inferred. • Correlation study of D9N variant of Lipoprotein Lipase (LpL) gene with CAD risk. In this study the D9N polymorphism and its correlation to lipid profile and subsequent CAD risk was studied. Project Summary- DNA isolation from patient and control blood samples, amplification of gene by Polymerase Chain Reaction, Cleaved Amplified Polymorphic Sequence of the amplified product, and analysis of the alleles by Agarose Gel Electrophoresis and Polyacrylamide Gel Electrophoresis.

Master'S Degree, Biotechnology, 4.0/4.0

Courses taken: GxP, Molecular Biology, Chemical Biology,Cell Biology, Immunology, Recombinant Protein lab,Ethical conduct of Research.

M.Tech (Integrated), Biotechnology, First Class

12 Th