• Performed multi-color flow cytometry analysis of tumor-antigen expression, copy number quantification, and binding affinities of therapeutic antibodies in cancer cell lines and primary T cells to support bispecific T-cell engager program.• Utilized cell engineering techniques to generate luciferase expressing and CRISPR/Cas9 knockout tumor cell lines for functional assays.• Executed Tumor - T cell co-culture assays to study cytotoxicity and cytokine production by T-cell engager molecules.• Performed ex-vivo mouse tumor/tissue processing for flow cytometry and gene expression analysis.

Tools and Techniques: Mammalian Cell Culture, SDS-PAGE, Western Blotting, Transfection, Fluorescence Microscopy, Hemocytometer Cell Counting, BCA Assay- Maintained HEK Blue cell lines through passaging and performed SEAP assay to test the effect of TNF- α on the IL-6 STAT signaling pathway.- Delivered RIC-3 DNA constructs in HEK wild-type cells by transfection, analyzed via fluorescent microscopy, extracted DNA, and performed BCA Assay for protein estimation in a three-member research team.- Separated protein extracts by SDS-PAGE and carries Western Blotting using anti-Myc antibodies.- Educated in six lab safety training including working in BL2 & BL3 labs, handling, storage & and disposal of radioactive and infectious material, and general lab inventory management.

Tools and Techniques - PCR (ddPCR & qPCR), ELISA, TU assay, Flow Cytometry, SEC-MALS, Batch and Perfusion Cell Culture, Transduction• Conducted bioprocess analytical assay for AAV8, AAV9, and LV viral vectors using ddPCR, TU-Assay, and ELISA for quantification of capsid and genome titer.• Performed batch and perfusion culture (ATF, TFDF), viral titer measurement, flow cytometry, and transfection using HEK 293A and VPC cells.

Tools and Techniques: DNA isolation(plant/Bacterial/Human/Fish), PCR, Agarose Gel Electrophoresis, Primer Designing, UV Nanodrop SpectrophotometryProject Title: Application of Molecular Markers- Amplified fish mt- DNA using MTCOX-1 gene-specific primers and explored Primer-BLAST for designing of primers.- Administered DNA extraction, PCR, and gel electrophoresis of plant, bacteria, and fish DNA, evaluated DNA sequence using Genetic Analyzer.- Guided 10 interns to conduct assays, provided initial analysis, optimized techniques to increase efficiency by 10%.

Tools and Techniques: Anti-oxidant Assay, Anti-haemolytic Assay, Colorimeter, Centrifugation, Student t-test- Evaluated antioxidant potential of four medicinal plants using biochemical assays- H2O2, TAC, FeCl3 with the determination of total phenols, alkaloids and flavonoids content, studied the in-vitro anti hemolytic capacity of plants in different solvent systems. - Performed descriptive statistics of data generated using ANOVA in MS Excel to analyze the significant differences and thus concluding the result.- Presented the work in International Conference on Recent Advances in Life Science held at Holkar Science College, Indore, and got an appreciation for the same.

Tools and Techniques: Biochemical Assay, Paper & Thin Layer Chromatography, Well and Disk diffusion method, Media & Buffer Preparation, Streaking and Plating TechniquesTitle: Phytochemical Screening and Antibacterial Activity of Medicinal Plants - Quantified secondary metabolites in four different solvent systems, conducted a battery of biochemical tests on Tinospora Cordifolia, Berberis Aristata, Cedrus Deodara, and Vitex Negundo to analyze their antibacterial activities.- Presented a Poster at an International Conference held at Indore & published a research article in Journal of Medicinal Plants Studies.

- Performed culturing of seeds, leaves, shoots, roots, anther, ovary, and protoplast fusion along with acclimatization of plants after a certain growth period - Studied the effects of growth hormones on plants in different conditions by modifying their concentration.