Mammalian Cell Culture.

Dealing with production of Therapeutic protein and mAbs by mammalian CHO cell lines and Hybridoma.• Operation and installation of single use bioreactor(100L,500L & 2KL)• Operation and installation of wave bioreactor.• Operation of SCADA and delta V.qqP• Operation of single use mixer.• Bioreactor readiness.• Assembly preparation of Filtration, Acid, Base, Antifoam, Inoculum transfer,Pooling, • Preparation of media, Media filtration, Media charging.• Filter integrity test by using Sartocheck 4.• Inoculums preparation at flask stage.• Operation and maintenance of CO2 shaker incubators.• Seed preparation from vial thaw to 100L bioreactor.• Aseptic sampling & dispensing.• CIP / SIP of media preparations vessel .• Operation of Autoclave.• Operation & maintenance of automated cell counter.• Operation & maintenance of Bioprofile medical analyzer, Blood gas analyzer.• Operation & calibration of pH and DO probe, osmometer, conductivity meter.• Maintaining GMP facility under state of control through daily monitoring.• Revising SOPs.• Preparation of facility for audit readiness.• Preparation of media, media assembly, acid, base assembly and filtration process. • BMR preparation and review.• Documentation as per GMP and GDP.

Capturing of disease, medicines, diagnosics results, and preparation of medical history of insured for giving health insurance.

i have done Six months of internship in biologics production department sponsored by BCIL a govt organization.Handling of SS bioreactor, CIP and SIP , Preparation of sampling assembly, probe calibration, media preparation media charging, Feed preparation, sampling and Feed addition.Documentation as per GMP and GDPFacility readiness.