James Broughton Email & Phone Number

Edinburgh, Gb

Edinburgh, Gb

• Investigated the bacterial DNA damage response and cellular physiology of E. coli under antibiotic combination treatment at the single-cell level.• Extensive experience using fluorescence microscopy and single-cell microfluidics. Developed novel experimental protocols and image/data analysis pipelines making use of Python, Matlab, and deep learning techniques. • Fabrication (soft lithography) of PDMS-based microfluidic chips for single-cell imaging. Some experience using photolithography to fabricate silicon wafers in cleanroom environment.• Molecular biology techniques: strain engineering, bioinformatics (NCBI Blast, pairwise alignments).• Effectively communicated findings through publications & presentations at multiple national and international conferences. Co-first authored publication: https://www.embopress.org/doi/full/10.15252/msb.202110441

• Supervision and demonstration of techniques to undergraduate students, marking of examinations, teaching course material. Courses demonstrated for: Microbiology, Genomes and Genomics

Edinburgh, Scotland, United Kingdom

Worked on a project studying the response of bacteria to DNA damage at the single-cell level using microscopy and microfluidics. Received training in microfluidics device fabrication and single-cell image analysis.

Stellenbosch, Western Cape, South Africa

• Managed a small research lab in the Department of Process Engineering.• Role involved maintaining equipment and procurement of consumables, training of engineering undergraduate and postgraduate students on basic microbiology techniques, lab equipment and safety.• Worked on a project related to the study and identification of hydrocarbon-degrading microorganisms isolated from hydrocarbon contaminated environmental samples. Involved 16s rRNA sequencing and GC-MS to evaluate hydrocarbon-degrading ability of isolates

Stellenbosch, Western Cape, South Africa

• Teaching and demonstration for chemistry and environmental engineering modules to engineering undergraduate students.• Invigilation and marking of examinations.

Stellenbosch, Western Cape, South Africa

• Thesis: Modelling biofilm development and biofilm-micropollutant interactions using an individual-based model.Experience:• Designed and built custom experimental set-up for the cultivation of wastewater-derived biofilms exposed to micropollutants (Diclofenac) in flow-cells, characterisation of microbial response via chemical effluent measurements (GC-MS)• Extensive experience with confocal laser scanning microscopy to quantify biofilm growth• Biofilm model development, validation, verification and application. Developed a mathematical model for batch microbial growth and parameterised the model using collected experimental data. Developed a biofilm model into the iDynoMiCS platform to simulate biofilm growth; comparing simulated biofilm quantitative parameters to those collected experimentally.• Informally supervised two undergraduate engineering students with final year research project.• Gave oral presentations at the WISA biennial conference and exhibition (2018) and the Stellenbosch University Water Institute symposium (2017)• Poster presentation of work at 17th International Symposium on Microbial Ecology in Leipzig, Germany

Durban, South Africa

• Supervision and demonstration of basic microbiology techniquess to undergraduate students, marking of examinations.

Durban, South Africa

• Tutoring first year biology students. One seminar per week with up to 20 students, prepared teaching material and mock tests

Doctor Of Philosophy - Phd, Cell/Cellular And Molecular Biology

Master Of Engineering - Meng, Chemical Engineering

Graduated Cum Laude. Thesis: Modelling biofilm development and biofilm-micropollutant interactions using an individual-based model

Bachelor Of Science Honours - Bschons, Microbiology, Graduated Summa Cum Laude

* Involved a 1 year independent research project. Thesis: Biofilm and quorum sensing inhibitory potential of green-synthesised bacterial.

Bachelor Of Science (Bs), Genetics, Microbiology

Activities and Societies: * Golden Key Honours Society * Class representativeGraduated Summa Cum Laude

Education record