James Murphy Email & Phone Number

Cambridge, England, United Kingdom

RNA-delivery modalities and technologies- Developed a high through put screening platform (DELiveri) for cell specific conjugated based RNA delivery.

Leeds, England, United Kingdom

Projects:• Targeting transfer RNA-derived fragments during Kaposi's sarcoma-associated herpesvirus infection.• Kaposi's sarcoma-associated herpesvirus induces virus specific specialised ribosomes during infection.Techniques:Isolation and culture of primary and cell line mammalian cells in BSL2 environments, maintenance and infection of a variety of human viral and bacterial pathogens, qRT-PCR, ribosome profiling, quantitative proteomics, nucleic acid extraction, gel… Show more Projects:• Targeting transfer RNA-derived fragments during Kaposi's sarcoma-associated herpesvirus infection.• Kaposi's sarcoma-associated herpesvirus induces virus specific specialised ribosomes during infection.Techniques:Isolation and culture of primary and cell line mammalian cells in BSL2 environments, maintenance and infection of a variety of human viral and bacterial pathogens, qRT-PCR, ribosome profiling, quantitative proteomics, nucleic acid extraction, gel electrophoresis, western blots, immunoprecipitation, confocal immunofluorescence microscopy, cell viability assays, cloning, subcellular fractionation, lentiviral transduction and stable cell line generation, transfection, and targeted RNA silencing.Publications:J. Murphy, E. Harrington, S. Schumann, E. Vasconcelos, T. Mottram, K. Harper, J. Aspden & A. Whitehouse. Kaposi’s sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs. Nature Communications., 14 (1), 300 Show less

• Thesis - Kaposi's sarcoma-associated herpesvirus induces virus specific specialised ribosomes during infection.• Techniques - Cell culture (BSL2), small fragment next Generation sequencing, RT-qPCR, western blotting, lentivirus expression systems, immunofluorescence microscopy, negative stain electron microscopy and, cell viability assays. • Publications - O Manners, JC Murphy, A Coleman, DJ Hughes, A Whitehouse. Contribution of the KSHV and EBV lytic cycles to tumourigenesis. Current… Show more • Thesis - Kaposi's sarcoma-associated herpesvirus induces virus specific specialised ribosomes during infection.• Techniques - Cell culture (BSL2), small fragment next Generation sequencing, RT-qPCR, western blotting, lentivirus expression systems, immunofluorescence microscopy, negative stain electron microscopy and, cell viability assays. • Publications - O Manners, JC Murphy, A Coleman, DJ Hughes, A Whitehouse. Contribution of the KSHV and EBV lytic cycles to tumourigenesis. Current opinion in virology 32, 60-70.1st Three Month Rotation• Project - Investigating the RNA binding properties of the Human Respiratory Syncytial Virus Transcriptional Antiterminator protein M2-1. • Supervisor - Assoc. Prof. Thomas Edwards• Techniques - Protein expression, protein purification, cloning, fluorescence anisotropy and, x-ray crystallography.• Publications - M Selvaraj, K Yegambaram, EJAA Todd, CA Richard, RL Dods, GM Pangratiou, CH Trinh, SL Moul, JC Murphy, J Mankouri, JF Éléouët, JN Barr, TA Edwards. The structure of the human respiratory syncytial virus M2-1 protein bound to the interaction domain of the phosphoprotein P defines the orientation of the complex. MBio 9 (6), e01554-18.2nd Three Month Rotation• Project - Synthesising Components for the Development of Protein-Protein Interaction Assays: eIF4G/eIF4E and M2-1/P protein. • Supervisor - Prof. Andrew Wilson• Techniques - Macromolecular organic chemistry and solid phase peptide synthesis. Show less

• Training and evaluating potential graduate recruits as diagnostic laboratory support scientists.

• Working within the UK Lighthouse Labs Network as part of the National Testing Program in response to the SARS-CoV-2 pandemic.• Sample processing for downstream qRT-PCR testing and uploading sample information into a Laboratory Information Management System (LIMS).

• Project - Human β-defensin-2 production upon viral and bacterial co-infection is attenuated in COPD.• Supervisor - Prof. David Proud, Airways and Inflammation, Snyder Institute for Chronic Diseases.• Techniques - Cell culture (BSL2), ELISA, real-time PCR, Western blotting, siRNA transfections and cell viability assays. • Publication - JW Arnason, JC Murphy, et al. Modulation of Airway Epithelial Human β-defensin-2 Expression following Human Rhinovirus and Pseudomonas aeruginosa… Show more • Project - Human β-defensin-2 production upon viral and bacterial co-infection is attenuated in COPD.• Supervisor - Prof. David Proud, Airways and Inflammation, Snyder Institute for Chronic Diseases.• Techniques - Cell culture (BSL2), ELISA, real-time PCR, Western blotting, siRNA transfections and cell viability assays. • Publication - JW Arnason, JC Murphy, et al. Modulation of Airway Epithelial Human β-defensin-2 Expression following Human Rhinovirus and Pseudomonas aeruginosa Co-infection in COPD. PloS one 12 (5). Show less

Leeds, United Kingdom

Project - Investigating the immunosuppressive properties of mesenchymal stem cells.Supervisor - Prof. Eileen Ingham.Techniques – Cell culture and RT-qPCR.

Bachelor Of Science (B.Sc.) Industrial (Ind.), Biology/Biological Sciences, General, Honours Class I

Activities and Societies: PASS Leader, Student Ambassador, Student Course Representative, Intramural Rugby, Leeds Marrow Volunteer Final.

A Levels - Abb, Gcse - A*, 7A, 2B, C

Activities and Societies: Rugby 1st, Rugby Captain 3rd, Combined Cadet Force (CCF) Non-commissioned Officer (NCO)