James Hurley Email & Phone Number

Cambridge Biomedical Campus, England, United Kingdom

Waltham

Cambridge, Ma

• Assay developmento Designed and developed an qPCR assay for the detection of a mRNA variant linked to metastatic castration-resistant prostate cancer (mCRPC).o Authored reviewed SOPs for performing assay, analytical validation and preparation of critical reagents.• Content engineo Developed a pipeline for biomarker discover in a number of disease states including kidney transplant rejection, lung cancer, Alzheimer’s and Parkinson’s.

Woburn, Ma.

Continued activities and responsibilities as manager at Biotrove into Life Technologies as a result of the purchase of Biotrove.Developing new applications for the high-throughput OpenArray real-time PCR platform. (including release of human and rodent microRNA OpenArray panels)

- Core team leader and technical lead for development of TaqMan qPCR technology for the OpenArray platform (a high-throughput real-time PCR platform capable of running up to 9000 reactions). - Led development of Sybr Green I-based qPCR for the OpenArray platform.- Co-ordinated R&D activities, developed protocols for verification and validation of new products.- Worked within cross-functional teams for NPIs.- Transfer of products and procedures to manufacturing teams- Authored SOPs and reports.- Led biology R&D team of 5 direct and indirect reports.- Worked with customers on proof-of-concept studies.- Regularly presented updates to peers and upper management.

- Developed homogeneous, multiplexed genotyping and haplotyping assays for the Luminex bead-based platform.- Programmed robotic liquid handling system to automate assays- Developed multiplex PCR protocols

- Developed novel, proprietary method for detecting single nucleotide polymorphisms (SNP).- Evaluated enzymes and DNA purification methods for novel genotyping technology- Performed assays for several internal and external customers.- Led development of template preparation and sequencing protocols that increased the throughput four-fold and decreased reagent usage 10-fold.- Authored several SOPs for high-throughput/low reaction volume template preparation and sequencing.- Supervised up to 4 reports providing product and services development

- Designed and performed tasks to carry out studies for a gene therapy approach to fight HIV-1 infection. Study progressed to animal experimentation phase. - Trained to work in BL3 facilities- Originated projects, involving gene therapy technology, to study the effects of HIV in the CNS using neural stem cells.

Ph.D., Biochemistry

Bsc, Biochemistry