Jamie Shirley, Phd Email and Phone Number
PhD scientist with 12 years of experience in immunology, gene therapy, and molecular biology. I am passionate about tackling scientific to medical challenges and advance the creation of medicines that make a positive impact on patient's lives. The majority of my scientific research experience has been focused on advancing AAV vector-based gene-replacement therapies to the clinic to treat rare inherited diseases with a primary focus on developing strategies to overcome unwanted immune responses. I also have experience in rational vector design strategies to improve tissue specificity and transduction efficiency. Currently I am working as a postdoctoral research fellow investigating cell-intrinsic functions of PD-L1 to overcome challenges associated with immune checkpoint blockade therapies for cancer.
Refinedscience
View- Website:
- refinedscience.com
- Employees:
- 31
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Scientist | Flow Cytometry SpecialistRefinedscienceAurora, Co, Us
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Project Manager | Assay DeveloperUniversity Of Colorado Anschutz Medical Campus Jan 2021 - PresentAurora, Colorado, United States• Elucidated cell signaling mechanisms of PD-L1 that contribute to immune cell trafficking and innate immune activation in the context of vaccination, infection, and cancer.• Mastered numerous molecular and cellular methods – high parameter multicolor flow cytometry, RT-qPCR, ELISA, Western blot, and immunofluorescence microscopy. • Optimized a cell-based luciferase assay for the quantification of type I IFN.• Overhauled a pre-existing protocol for differentiating hematopoietic stem cells into DCs and successfully increased yield from ~50% to 80%.• Leveraged communication and interpersonal skills to train and manage technicians and students. -
Project Team Lead | Subject Matter Expert | Data ManagerUniversity Of Massachusetts Medical School Oct 2019 - Dec 2020Shrewsbury, Massachusetts, United States• Managed and led a multidisciplinary industry-academia collaborative project testing a novel AAV gene therapy vectors containing RNAi elements that regulated expression levels.• Created and optimized a cell-based neutralizing antibody assay for AAV capsid. • Developed a flow-based method to sort microglia from mouse brain tissue and evaluate cell specific AAV transduction efficiency by RT-qPCR.• Utilized barcoded AAV capsid libraries to identify genetic variants with enhanced transduction efficiency and tissue selectivity.• Organized, trained, and led a small team of students and technicians. -
Project Manager | Data Analyst | Scientific CommunicatorUniversity Of Florida College Of Medicine Aug 2014 - Aug 2019Gainesville, Florida, United States• Designed and executed a research project aimed at developing strategies to overcome unwanted T cell responses to AAV vector-based gene replacement therapies.• Optimized a cell-based colorimetric assay to quantify T cell receptor signaling.• Developed an expertise in multi-color flow cytometry, immunohistochemistry, and cell-culture.• Engineered and tested novel gene cassettes that reduced the immunogenicity of AAV vectors.• Presented my work at national conferences and prepared written grant applications and scientific manuscripts for publication. -
Course Creator | InstructorUniversity Of Florida College Of Medicine May 2016 - Jul 2017Gainesville, Florida, United States• Developed and implemented a 7-week course on the topic of Immunology & the Principles of Vaccine Design for exceptional high-school students interested in STEM fields from the state of Florida.• Created hands on learning activities.• Evaluated student’s understanding of learning objectives.
Jamie Shirley, Phd Education Details
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Immunology And Gene Therapy
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Molecular Biology And Microbiology
Frequently Asked Questions about Jamie Shirley, Phd
What company does Jamie Shirley, Phd work for?
Jamie Shirley, Phd works for Refinedscience
What is Jamie Shirley, Phd's role at the current company?
Jamie Shirley, Phd's current role is Scientist | Flow Cytometry Specialist.
What schools did Jamie Shirley, Phd attend?
Jamie Shirley, Phd attended University Of Florida College Of Medicine, Auburn University.
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