Jared Dennis Email & Phone Number

Durham, Nc, Us

Durham, Nc, Us

Ludwigshafen, De

Ludwigshafen, De

Serve as team leader for enzyme discovery, library construction, and bioresources management. Design, plan, and construct high-throughput gene libraries for protein engineering efforts. Develop and utilize rational and semi-rational design and directed evolution approaches for improving performance and manufacturing robustness of enzymesImplement novel directed evolution library processes for creatively exploring new sequence space and enzyme diversity by integrating synthetic biology techniques, lab automation, NGS sequencing, and functional enzyme screeningWork to develop automated and semi-automated pipelines for cloning,gene-assembly, and heterologous protein expression for bacterial and fungal expression platforms. Collaborated with automation and bioinformatics departments to scout, procure and validate state-of the-art laboratory automation platformsProvide lab consultation, lab support, and dossier reports to Regulatory Affairs team for molecular-biology related aspects of enzyme commercialization. Activities included planning and executing sequence based microbial strain identification, plasmid-based genetic stability studies, and other microbiological characterization of production strains in accordance with relevant regulatory agency guidelines

Ludwigshafen, De

Integrated Molecular Biology research tools with Riffyn for achieving a global experiment data capture platform. Efforts aided digitalization efforts and provided experiment design, workflow tracking, and data analysis capabilityDesign and construction of gene variant libraries using novel high-throughput directed-evolution techniques for functional screening of enzymes. Created and validated a novel PCR-based assay for the detection and quantification of genetic markers in formulated commercial enzyme preparations, replacing the pre-existing method and significantly reducing resources and effort required by for the Quality Control department. Oversaw biological sample management, including microbiological samples, gene libraries, and a variety of environmental samples.Provided extensive genetic characterization of enzyme production strains for regulatory approval necessary for the commercialization of industrial enzymes. Created written reports detailing morphological, physiological, and genetic characterization of production organisms. This included evaluating the stability and mobility of associated genes and plasmids within microbial production strains.Responsible for molecular biology laboratory management; evaluating and procuring supplies, reagents, and capital equipment for research needs.

Ludwigshafen, De

Designed and generated gene libraries using whole gene (SSM) site-saturation mutagenesis, iterative SDM, epPCR, gene-shuffling, and other techniques for diversity generation. Ensured high quality library standards were met and transformed and prepared libraries for screening in microbial hosts

San Diego, Ca, Us

Established and led a five person multidisciplinary team responsible for the small scale production (<10L), recovery, characterization, and archival of numerous industrial enzymes. Managed a team of junior scientists responsible for providing core cell and molecular biology support for enzyme development including gene cloning and subcloning, directed evolution, gene sequencing, plasmid engineering, and small scale expression optimization and functional characterization via diverse biochemical assaysSuccessfully managed and coordinated contract research efforts with industrial biotechnology partners; established necessary financial budgets, human resources, and project timelines with deliverables. Served as in-house technical expert on proprietary evolution technology. Responsible for establishing project budgets, timelines, and personnel requirements for numerous projects.Responsible for evaluating and procuring supplies, reagents, and capital equipment for laboratory research.Managed in-house DNA sequencing team responsible for Sanger sequencing and data analysis

San Diego, Ca, Us

Developed and optimized a novel gene reassembly technology for creating large libraries (>20,000 variants) of combinatorial site-specific mutations. Collaborated with Verenium’s Intellectual Property team providing data and technical expertise on a subsequent patent application.Designed and constructed high-throughput site-saturation mutagenesis libraries and combinatorial reassembly libraries for enzyme product candidates; operated high-throughput automation equipment to prepare and array variants for subsequent screening efforts.Responsible for phage monitoring/detection efforts supporting industrial and lab-scale fermentations; designed genetic selections for the isolation and characterization of phage-resistant bacterial expression strains.

San Diego, Ca, Us

M.S., Biological Sciences

B.S., Marine And Freshwater Biology