Chen-Jung (Jason) Lee Email & Phone Number

Protein conjugation

Technical lead in the team of analytical and formulation science focusing on protein analysis from titer measurement of crude product, property characterization of final product, to protein stability study.· Assay development and troubleshooting· Assess the feasibility of client’s project, design the assays, deliver the results, and answer client’s questions· Write GMP SOPs for analytical methods

Hayward, Ca

· Mainly work on antibody and protein analytics: HPLC, DSC, DSF, DLS/SLS, ELISA, BLI· HPLC: Protein purity by size exclusion chromatography and reverse-phase chromatography· DSC/DSF (TA Instruments NanoDSC, Unchained Labs UNcle): Protein thermostability· DLS/SLS (Unchained Labs UNcle): Size distribution of protein solution· ELISA: Method development of direct, indirect and sandwich ELISA for quantification and affinity measurement· BLI (Octet and Gator): Affinity measurement, off-rate ranking, epitope binning· Other protein characterization techniques: CE-SDS, SDS-PAGE, cIEF· Be trained to perform GMP projects

Cleveland/Akron, Ohio Area

· Assisted with professor Gang Cheng in designing a novel organic conductive polymer with controllable anti-fouling and antimicrobial properties for a patent· Maintained, troubleshot, and validated equipment as appropriate· Maintained lab notebooks and kept complete and accurate records of experimental methods and data, prepared data for presentation and wrote technical reports, familiar with GMP/GLP and ICH/GCP guidelines, submitted manuscripts for publicationProject I: Study of Structure-Function Relationships of Tertiary Amine-Based Polycarboxybetaineo Designed and synthesized a novel biomaterial integrated with antifouling, antimicrobial and buffering capability useful for wound healing (to maintain the pH value of wound surface and provide a protection from the attachment of undesired substances)Project II: Ionic Conductivity of Polyelectrolyte Hydrogelso Investigated systematically ionic conductivity and swelling behaviors of various polyelectrolyte hydrogels (cationic/zwitterionic/anionic) to provide an insight into the effect of polyelectrolyte side chain on ionic transport for a better decision in choosing suitable polyelectrolytes for a broad spectrum of applications.Project III: Electroactive PolySBEDOT and its derivatives with Controllable Antifouling and Antimicrobial Propertieso Designed and synthesized a series of organic electrically conducting polymers with high resistance to the attachment of protein, cells, and bacteria and low interfacial impedance for many long-term bio-electrochemical applications such as implantable biosensing, robotic prostheses and biofuel cell, which required high performance conducting materials with excellent antifouling property/biocompatibility at complex bio-interfaceso Invented collaboratively the first zwitterionic conductive material (PSBEDOT) based amperometric glucose biosensor by simple one-step fabrication with high sensitivity, high selectivity and low detection limit (0.01mM/mL)

Doctor Of Philosophy - Phd, Chemical Engineering

Bachelor Of Engineering - Be, Chemical Engineering