June Javens-Wolfe Email & Phone Number

Indianapolis, Indiana, United States

• Conducting laboratory research to support lead generation of antibody and small molecule therapeutics and tools• Developing and optimizing cell-based assays to screen therapeutic compounds• Cloning, expressing, and purifying novel antibodies• Determination of antibody titer and KD analysis using BLI• Providing training for laboratory instruments (FACSAria, Operetta high content imager, AKTA Pure FPLC, Namocell Hana single cell dispenser) and coordinating service calls and PMs

Indianapolis, Indiana Area

• Conducting laboratory research to support lead generation of antibody and small molecule therapeutics and tools• Establishing a B-cell cloning platform involving single cell sorting of antigen-specific B-cells from PBMCs, inducing B-cell expansion, and cloning antibody variable domains• Developing a workflow to clone, purify plasmid and transfect CHO cells in small batches at microplate-scale• Managing of and providing training for laboratory instruments (FACSAria and Operetta… Show more • Conducting laboratory research to support lead generation of antibody and small molecule therapeutics and tools• Establishing a B-cell cloning platform involving single cell sorting of antigen-specific B-cells from PBMCs, inducing B-cell expansion, and cloning antibody variable domains• Developing a workflow to clone, purify plasmid and transfect CHO cells in small batches at microplate-scale• Managing of and providing training for laboratory instruments (FACSAria and Operetta high content imager)• Ordering supplies and assisting with set up of new laboratory• Assisting with summer research projects for interns Show less

Indianapolis, Indiana Area

Coordinated small molecule screening projects between Eli Lilly and external partners (early drug discovery). - Documented research plans and workflows in collaboration with external investigators - Created summaries of project activities and progress, maintained records - Analyzed high throughput screening data and evaluated dose-response curves - Interfaced with numerous groups (IT, sample management, quantitative biology, etc) to manage project progress and… Show more Coordinated small molecule screening projects between Eli Lilly and external partners (early drug discovery). - Documented research plans and workflows in collaboration with external investigators - Created summaries of project activities and progress, maintained records - Analyzed high throughput screening data and evaluated dose-response curves - Interfaced with numerous groups (IT, sample management, quantitative biology, etc) to manage project progress and improve internal workflows Show less

Indianapolis, Indiana Area

- Tested biologic drug samples for potency using ELISAs and reporter gene cell-based assays in a GMP environment - Cultured 11 different mammalian cell lines - Assisted client in investigation, troubleshooting, and development of bioassay methods

Greencastle, In

- Instructor of record for Microbiology (BIO 250)- Responsible for two sections, each with three hours of lecture and three hours of laboratory activities a week- Created and piloted experiments for student lab assignments- Coordinated lab activities with lab manager- Developed and implemented course syllabus, lectures, assignments, and exams

Bloomington, Indiana

Instructor of record for Molecular Genetics Laboratory (BIOL-M485)Guided college juniors and seniors through semester long molecular genetics research projectImproved and piloted experiments for student lab assignmentsDeveloped and implemented course syllabus, lectures, assignments, and examsEvaluated student performance Managed teaching assistantOrdered supplies and reagents

Bloomington, Indiana Area

• Conducted independent microbial molecular biology research project • Authored experimental protocols• Trained and mentored other lab members in learning techniques• Prepared research paper for publication

Bloomington, Indiana Area

• Conducted independent microbial molecular biology research project • Authored experimental protocols• Coordinated assignments of lab jobs, helped develop guidelines for lab operations/procedures• Trained and mentored other lab members in learning techniques• Delivered and critiqued professional presentations• Wrote and critiqued grants and papers for submission to peer reviewed journals

Bloomington, Indiana

Instructor of record for Microbiology lecture course (BIOT 221)Developed and implemented course syllabus, lectures, assignments, and examsEvaluated student performance

B.A., Biology And French

Ph.D., Microbiology