Jayesh Tambe Email & Phone Number

Mumbai, Mh, In

Navi Mumbai, Maharashtra, India

Structural Protein Purification: I specialize in isolating highly pure structural proteins using chromatographic techniques and purification methods. These proteins are vital for maintaining cell and tissue structure, with applications in cell biology, biophysics, and structural biology.Project Management: I lead projects related to structural protein purification. This involves managing timelines, allocating resources, and ensuring project goals and milestones are achieved. I coordinate across teams, track progress, and address any project challenges, maintaining open communication and regular reporting.Design of Experiments (DOE): I employ DOE to optimize structural protein purification. Through systematic experimentation and statistical analysis, I refine purification processes, enhancing protein yield and quality. This ensures that the final purified proteins meet required specifications for downstream applications and research.

Bengaluru, Karnataka, India

As a Senior Research Associate at Syngene International Ltd since September 2020, my primary roles and responsibilities include:Purification of therapeutic proteins, including monoclonal antibodies, using various chromatographic techniques such as affinity, gel filtration, hydrophobic interaction, and ion-exchange chromatography.Optimization of expression and purification processes for recombinant proteins in host systems like E. coli, utilizing chromatographic systems.Analysis of protein expression and purification through techniques such as Western Blot.Process development and optimization for the purification of antibodies.Performing qualitative and quantitative analysis of purified proteins using methods like A280, Bradford, BCA, SDS-PAGE gel, and SE-HPLC.Collaboration and coordination with the analytical, upstream, and molecular biology teams to determine the appropriate methods for protein purification.Providing weekly project reports to the manager, summarizing milestones, project goals, scope, success, and any issues encountered.Ensuring contemporaneous documentation, preparation of technical reports, and delivering presentations as required.

India

As a Trainee Executive Officer at Serum Institute of India from August 2019 to August 2020, my roles and responsibilities included:Downstream Process Development: I was involved in the development of downstream processes in a cGMP (current Good Manufacturing Practice) laboratory. This specifically focused on the purification of CRM197, a protein used in vaccines. The purification techniques I worked with included hydrophobic interaction chromatography, dye ligand chromatography, reverse phase chromatography, and ion exchange chromatography. These techniques help separate and purify the target protein from impurities.Tangential Flow Filtration: I have performed protein purification using tangential flow filtration (TFF) techniques. TFF is a filtration method used to concentrate and purify proteins by selectively passing them through a filter while retaining larger impurities and contaminants.Qualitative and Quantitative Analysis: I have conducted qualitative and quantitative analysis of the purified proteins using various methods. A280 refers to the measurement of protein concentration using absorbance at 280 nm, which is a common method for estimating protein concentration. SDS-PAGE gel (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) is a technique used to separate and analyze proteins based on their molecular weight.Contemporaneous Data Recording: I have maintained contemporaneous data recording in Batch Manufacturing Records (BMRs). BMRs are documents used to record the steps, parameters, and results of each batch of production or purification. This helps ensure traceability and adherence to regulatory standards.SOP Preparation and Revision: I was responsible for the preparation and revision of Standard Operating Procedures (SOPs). SOPs are detailed instructions that outline the step-by-step processes and protocols to be followed for various activities. They provide guidance on how to perform tasks consistently and maintain quality standards.

Pune Area, India

As an Academic Intern at Lupin Ltd in Pune from August 2018 to February 2019, my primary responsibility was the execution of downstream processing of antibiotics using preparative chromatographic methods. Here's a breakdown of my role:Downstream Processing: I was involved in the downstream processing of antibiotics. Downstream processing refers to the purification and separation of the target compounds from crude mixtures or fermentation broths. In this case, I specifically worked with antibiotics.Preparative Chromatographic Methods: I have executed preparative chromatographic methods for the purification of antibiotics. Preparative chromatography is a technique used to separate and purify target compounds on a larger scale compared to analytical chromatography. It involves using chromatographic columns and techniques such as high-performance liquid chromatography (HPLC) or flash chromatography.My role likely included the following tasks:Column Packing: Preparation and packing of chromatographic columns with the appropriate stationary phase or resin to facilitate separation.Sample Loading: Application of the antibiotic-containing sample onto the column for purification.Elution: Implementation of the appropriate elution conditions to selectively elute the desired antibiotic compounds from the column while leaving impurities behind.Collection and Analysis: Collection of purified antibiotic fractions and subsequent analysis to ensure the quality and purity of the isolated compounds. Analysis may involve techniques such as thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), or other analytical methods.Optimization and Troubleshooting: Collaborating with the team to optimize purification protocols and troubleshoot any issues that may arise during the downstream processing.

Master Of Technology - Mtech, Bioprocess Technology

Bachelor Of Pharmacy - Bpharm, Pharmacy

Hsc, Science

Ssc, Science