Jayne Schaubhut Email & Phone Number

Scottsdale, Arizona, US

Bothell, Washington, US

Kagoshima, Kagoshima, JP

• Run flow cytometry experiments according to GLP regulations and as described in the appropriate oversight documents (ex: SOPs, Study Protocol, and Analytical Study Plan).
• Critically analyze study or project data to compile into reports for client sponsored GLP and non-GLP preclinical drug studies.
• Collect and review analytical biology data as the Contributing Scientist or Principal Investigator.
• Write technical documents such as Analytical Study Plans, SOPs, study forms and data reports
• Develop and implement new assays in the flow cytometry group and projects to support internal and client sponsored studies.
• Respond to inquiries from clients regarding status of their project or flow cytometry capabilities.

Thousand Oaks, CA, US

• ONCOLYTIC VIRUS (CANCER IMMUNOTHERAPY) 2012-2014
• Created IncuCyte two color assay to examine the complex viral cytotoxic effect on cancer cells using fluorescent reporter antibodies and transducing cell lines to express infrared protein.
• Determined effectiveness of Oncolytic Virus on pediatric cancer cell lines in vitro and in vivo. In vitro readout monitored ATP activity (cell titer glo); while in vivo methods were measured by tumor regression. These.
• Increased thru-put for flow cytometry primary T-cell assay by utilizing less toxic dyes that were read by separate lasers. Collaborated with colleagues and reviewed literature to create protocol with minimal supervision.
• Investigated biodistribution of BiTE molecule in tumor-bearing, humanized, intra-peritoneal T-cell mouse model. T-cells were activated, expanded, and implanted in mice. BiTE was dosed intra-peritoneal, then tumor.
• Looked to as a person to quickly understand new computer software and easily adopted techniques. Often tasked to train colleagues in innovative procedures with an eye on efficiency.

Thousand Oaks, CA, US

• ONCOLOGY LARGE MOLECULE (MACROPHAGE ANTIBODY)
• Influenced the selection of IL-34 antibody used in IHC and ELISA. Antibodies screened by multi-colored flow cytometry and Western blot to attain an understanding of TAM biology. Presented and discussed results with.
• Established tumor ex vivo protein extraction method for syngeneic and xenograft mouse models. Utilized this method to correlate protein levels to tumor regression after treatment with a macrophage antibody..
• Investigated differences in tumor regression results when model was moved to CRO. Microarray results revealed CRO was given a disparate cell line.
• Used literature to develop state-of-the-art method of screening multiple cells lines for receptor of interest.
• Collaborated with colleague to write study report on the effect of a macrophage antibody against an established tumor-bearing mouse model.

Thousand Oaks, CA, US

• Provided project assistance by the creation of routine buffers and media, completing common bioassays and ELISAs.
• Cataloged all cell lines for the department in order to establish a computerized searchable database.
• Established stocking levels and easy to follow inventory location maps.
• Contributed end-user feedback to Radiation Safety Committee to establish site-wide Standard Operating Procedures (SOPs).
• Generated business continuity plan for Oncology Department.
• Received BRAVO award for successfully outfitting new lab with appropriate equipment and supplies in time for employee on-boarding during high-expansion period.

Thousand Oaks, CA, US

• Care for Oncology laboratory; including stocking, ordering and media/buffer preparation.
• Established in vitro assay radiation room.
• Coordinate maintenance of laboratory equipment with Engineering department.

• Responsible for maintaining glassware facility.
• Member of radiation survey team, completed radiation safety training.
• Used problem solving skills to respond to all levels of research problems, i.e. Lab Assistant to Senior Scientist.
• Prepared buffers and media according to protocols.