Collaborative Associate Scientist with a can-do attitude, intellectual drive, and effective written and verbal communication skills. Ten years biotechnology experience delivering results in vitro, in vivo and ex vivo focused on oncology and immunology. Currently focused on delivering quality, pre-clinical, GLP flow cytometry data in a CRO organization. Disseminated data analysis summary via email, presentations, technical reports and electronic lab notebook entries.PROFICIENCIESMolecular and Cellular Biology: • ELISA• Functional cell-based assay development (proliferation, cell death, etc.)• Luminex (multiplex, MSD)• HCS Fluorescence Imaging• Live cell fluorescence imaging (IncuCyte FLR and ZOOM)• Western blot • SDS-PAGE • RNA purification • Mammalian and primary cell tissue culture• DNA transfection/transduction• Flow cytometry staining and analysis • Human T cell proliferation assays • Mouse, cyno and human tissue homogenization to whole cell suspension or proteins In vivo Biology:• Skilled in numerous mouse dosing techniques including oral gavage, i.p., s.c. and more.• Expert at blood collection methods including saphenous, tail nick and retro-orbital.• Experience in mouse models including: proof-of principle models, efficacy models, and tolerability studies with a large focus on tumor models. • Responsible for all aspects of in vivo tumor models including study design, animal dosing, tissue collection, data collection and data analysis. Computer Software:• Graphpad Prism- graphing and statistical analysis• Microsoft Office- Outlook, Word, Excel, PowerPoint• IncuCyte ZOOM and FLR • Li-Cor • Cellomics ArrayScan analysis • FCS Express• FlowJO • Electronic Notebook• Evernote