Jay Sharma Email & Phone Number

San Diego, Ca, Us

Basel, Baselstadt, Ch

- Platform development to enable in vivo HSC editing for cell/gene therapy - Immunogenicity studies to establish feasibility of an adenovirus concept - Preparation and CRISPR-CAS9 editing of embryonic stem cells for microinjections into blastocyst

San Diego, California, Us

-• Leading the research team on Phase 1/2 clinical trials to enable six patients with cystinosis receive hematopoietic stem cell (HSC) - based therapy-Testing and validating drug products for clinical trial patients followed by submission of official reports and data summaries-Liaising with external academic and industry collaborators to effectively disseminate data, transfer technology and plan a long-term follow-up study-Using immunological techniques (including ELISA, flow cytometry, and immunohistochemistry), in vitro tissue cell culture, and bioassay development using primary mouse/human cells and engineered cell lines-Conducting immunogenicity studies to determine the effect of gene therapy on macrophage activation and differentiation from patient blood -Developing in vitro cell-based assays to improve HSC-mediated gene-editing via lentivirus transduction using a biological adjuvant, lentiboost, in human CD34+ HSCs-Performing isolation, transduction and transplantation of HSCs isolated from mouse bone marrow and human peripheral blood using magnetic beads kit-Establishing and applying routine maintenance, calibration & training of BD C6 Accuri flow cytometer-Assisting scientists in developing HSC-based bone marrow transplant gene therapies against Friedreich’s ataxia (CRISPR-based), Danon disease and Alzheimer’s disease mouse models publishing original findings in high-quality journals

La Jolla, Ca, Us

-Led pre-clinical research trial of Cystinosis from conception to execution while complying with protocols and SOP requirements as per Good Laboratory Practice guidelines-Collected and reviewed pre-clinical study data, source documentation, and quality-assured data files to verify the accuracy and completion of Investigational New Drug application for Cystinosis-Developed and optimized assays involving mouse models of Danon disease and Cystinosis-Isolated and cultured primary immune cells (T-cells, B-cells, and myeloid cells) from mouse tissues and assessed lentivirus vector integration-Developed filter-based dot-blot assay to detect immune response against CTNS-expressing cells-Determined lentivirus transduction efficiency in mouse blood and human stem cells via flow cytometry-Performed ELISAs and diagnostic tests to evaluate kidney function to test the efficacy of drug product in mice-Optimized cell-based assays to study interaction between fibroblasts and macrophages and performed in vitro colony forming unit assays to study the effect of gene therapy on hematopoiesis of human HSCs -Performed safety area coordinator and lab managerial roles mainly including maintaining lab safety and compliance, personnel training records, and inventory management

La Jolla, Ca, Us

-Designed and optimized cell-based and mouse-based assays to investigate lead therapeutics to treat type-2 diabetes and rheumatoid arthritis mouse model-Developed proficiency with general skills of molecular cell biology, such as DNA manipulation, RNA isolation, electrophoresis, gel staining, PCR-Gained familiarity with immune phenotyping, using flow cytometers such as LSR Fortessa II and cell sorting on BD FACS Aria II/III-Performed ELISAs to estimate cytokine levels in immune cells, Apoptosis assay, spreading and migration assay, and Western blotting

La Jolla, Ca, Us

-Investigated the mechanisms of hematopoietic stem cell-mediated therapy involved in the treatment of Cystinosis, a lysosomal storage disorder -Evaluating the physiological and genomic mechanisms involved in stem cell recruitment and tissue repair process in the mouse model of Cystinosis-Designed and optimized various in vitro assays to study the cell-to-cell transfer of functional cystinosin protein -Obtained different plasmid constructs by site-directed mutagenesis and sub-cloning for lentivirus vector preparation, eventually obtaining stable cell lines by transduction-Developed shRNA-based cell models to study the mechanisms of stem cell-mediated tissue repair process in Cystinosis

Los Angeles, Ca, Us

The Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, located on the University of Southern California’s Health Sciences Campus. Here, the investigators are exploring the normal mechanisms that build, maintain and repair our body systems, to develop knowledge-based approaches for regenerative medicine. Investigators are exploring kidney, liver, neural, blood, cardiovascular, skeletal and skin disease models. The center serves as a hub for USC Stem Cell, which connects researchers in stem cell biology and regenerative medicine across USC.• Attended an extensive training program on embryonic stem cell (ESC)/human pluripotent stem cell (hPSC) culture techniques and their maintenance • Efficiently acquired hands-on training for plating dissociated hPSC's, primary mouse embryonic fibroblast (MEF) isolation, MEF feeder layer production, and quality control testing in hPSC cultures

Camarillo, Ca, Us

• Optimized the growth conditions of mouse Mesenchymal stem cells (mMSCs) in 3D fibrin scaffold constructed of varying concentrations of fibrinogen and thrombin• Employed live/dead assay and fluorescence microscopy to check the cell proliferation rate of mMSCs

Camarillo, Ca, Us

• Checked the effect of over-expression of cyclin-D1 gene on cell proliferation of Chinese Hamster Ovary (CHO) cells.• CHO cells were transfected with cyclin-D1 gene using Lipofectamine-2000 and cell proliferation was checked by using MTS assay

In

Blossoming since 2008, Disha Life Sciences Pvt. Ltd. is Renowned name in Gujarat offering at par excellence in academics and nourishing youth for tomorrow's challenge. It is a platform where a student grows to scholar and a scholor grows to a veteran. Disha Life Sciences is equipped with state of art laboratories for Biotechnology, Molecular Biology, Microbiology, Fermentation and Plant Tissue Culture.• Performed molecular characterization of E.coli using various biochemical tests, Gas Chromatography and randomly amplified polymorphic DNA approach• Laboriously practiced basic molecular biology techniques such as polymerase chain reaction, DNA cloning and gel electrophoresis • Proficiently utilized basic microbiology techniques such as inoculating agar plates, growing bacteria on selective media, isolating an organism from the environment, enumerating bacteria using serial dilution, plate counts and spectrophotometer

Pimpri, Pune, Maharashtra, In

• Investigated the efficacy of aqueous Aloe vera extract on serum profile and histological examination of pancreas in alloxan-induced diabetic rats. • Determined a novel method for treatment of diabetes in rats followed by statistical data analysis of glucose, protein and lipid levels in rats treated from diabetes.• Anesthetized and isolated pancreas from rats, and performed histological examination to check for the efficiency of herbal treatment in diabetic rats.

Ms, Biotechnology & Bioinformatics

Ms, Biological Sciences

B.Tech, Biotechnology