Jay Sharma Email and Phone Number
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Hi, I'm Jay, a researcher with over 10 years of experience in cellular/molecular biology and immunology, with a focus on stem cells and gene therapy. I'm based in San Diego, CA, and have played a part in launching the first human clinical trials for stem cell and gene therapy treatments for Cystinosis, backed by $17.2 million in grants.I've been fortunate to receive awards, scholarships, and fellowships totaling over $75,000 for my work. I've also co-authored 6 journal publications and served as a peer reviewer for more than 8 international journals.I moved to the US from India in August 2011 to pursue an MS in Biotechnology at California State University, Channel Islands. At the time, my career goals were still taking shape, and I wish I had better guidance on job prospects, personal finance, and visa issues. Over the past 14 years, I’ve learned so much and I’m excited to share my insights about life in the US.I initially aimed for a PhD and focused on academic jobs, but after two years in a PhD program, I realized it wasn't the right path for me. It took another five years to successfully transition to industry in April 2023, navigating various visas (F1, J1, O1, EB2, EB1) along the way.-----------------------------------------------------------------------------------------------------Topics I Can Help With: ▪ Transitioning from academia to industry ▪ Navigating visa challenges on the way ▪ Building an EB1A/EB2 profile I’m passionate about helping others on their journey! :)Please fill out the form if you'd like to schedule a free 1:1 video consultation -https://forms.gle/qxY9k2qYHTvn2wQz9
University Of California, San Diego - School Of Medicine
View- Employees:
- 31563
- Company phone:
- +41 61 324 11 11
- Company email:
- eric.althoff@novartis.com
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Staff Research Associate IiiUniversity Of California, San Diego - School Of MedicineSan Diego, Ca, Us
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Senior Scientist IiNovartis Mar 2023 - PresentBasel, Baselstadt, Ch- Platform development to enable in vivo HSC editing for cell/gene therapy - Immunogenicity studies to establish feasibility of an adenovirus concept - Preparation and CRISPR-CAS9 editing of embryonic stem cells for microinjections into blastocyst -
Staff Research Associate IiiUniversity Of California, San Diego - School Of Medicine Aug 2018 - Mar 2023San Diego, California, Us-• Leading the research team on Phase 1/2 clinical trials to enable six patients with cystinosis receive hematopoietic stem cell (HSC) - based therapy-Testing and validating drug products for clinical trial patients followed by submission of official reports and data summaries-Liaising with external academic and industry collaborators to effectively disseminate data, transfer technology and plan a long-term follow-up study-Using immunological techniques (including ELISA, flow cytometry, and immunohistochemistry), in vitro tissue cell culture, and bioassay development using primary mouse/human cells and engineered cell lines-Conducting immunogenicity studies to determine the effect of gene therapy on macrophage activation and differentiation from patient blood -Developing in vitro cell-based assays to improve HSC-mediated gene-editing via lentivirus transduction using a biological adjuvant, lentiboost, in human CD34+ HSCs-Performing isolation, transduction and transplantation of HSCs isolated from mouse bone marrow and human peripheral blood using magnetic beads kit-Establishing and applying routine maintenance, calibration & training of BD C6 Accuri flow cytometer-Assisting scientists in developing HSC-based bone marrow transplant gene therapies against Friedreich’s ataxia (CRISPR-based), Danon disease and Alzheimer’s disease mouse models publishing original findings in high-quality journals -
Staff Research Associate IiUniversity Of California, San Diego Sep 2014 - Mar 2016La Jolla, Ca, Us-Led pre-clinical research trial of Cystinosis from conception to execution while complying with protocols and SOP requirements as per Good Laboratory Practice guidelines-Collected and reviewed pre-clinical study data, source documentation, and quality-assured data files to verify the accuracy and completion of Investigational New Drug application for Cystinosis-Developed and optimized assays involving mouse models of Danon disease and Cystinosis-Isolated and cultured primary immune cells (T-cells, B-cells, and myeloid cells) from mouse tissues and assessed lentivirus vector integration-Developed filter-based dot-blot assay to detect immune response against CTNS-expressing cells-Determined lentivirus transduction efficiency in mouse blood and human stem cells via flow cytometry-Performed ELISAs and diagnostic tests to evaluate kidney function to test the efficacy of drug product in mice-Optimized cell-based assays to study interaction between fibroblasts and macrophages and performed in vitro colony forming unit assays to study the effect of gene therapy on hematopoiesis of human HSCs -Performed safety area coordinator and lab managerial roles mainly including maintaining lab safety and compliance, personnel training records, and inventory management -
Research Laboratory TechnicianLa Jolla Institute For Allergy And Immunology Dec 2013 - Aug 2014La Jolla, Ca, Us-Designed and optimized cell-based and mouse-based assays to investigate lead therapeutics to treat type-2 diabetes and rheumatoid arthritis mouse model-Developed proficiency with general skills of molecular cell biology, such as DNA manipulation, RNA isolation, electrophoresis, gel staining, PCR-Gained familiarity with immune phenotyping, using flow cytometers such as LSR Fortessa II and cell sorting on BD FACS Aria II/III-Performed ELISAs to estimate cytokine levels in immune cells, Apoptosis assay, spreading and migration assay, and Western blotting -
Cirm Research FellowUc San Diego Oct 2012 - Sep 2013La Jolla, Ca, Us-Investigated the mechanisms of hematopoietic stem cell-mediated therapy involved in the treatment of Cystinosis, a lysosomal storage disorder -Evaluating the physiological and genomic mechanisms involved in stem cell recruitment and tissue repair process in the mouse model of Cystinosis-Designed and optimized various in vitro assays to study the cell-to-cell transfer of functional cystinosin protein -Obtained different plasmid constructs by site-directed mutagenesis and sub-cloning for lentivirus vector preparation, eventually obtaining stable cell lines by transduction-Developed shRNA-based cell models to study the mechanisms of stem cell-mediated tissue repair process in Cystinosis
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Research TraineeUniversity Of Southern California Jul 2012 - Jul 2012Los Angeles, Ca, UsThe Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, located on the University of Southern California’s Health Sciences Campus. Here, the investigators are exploring the normal mechanisms that build, maintain and repair our body systems, to develop knowledge-based approaches for regenerative medicine. Investigators are exploring kidney, liver, neural, blood, cardiovascular, skeletal and skin disease models. The center serves as a hub for USC Stem Cell, which connects researchers in stem cell biology and regenerative medicine across USC.• Attended an extensive training program on embryonic stem cell (ESC)/human pluripotent stem cell (hPSC) culture techniques and their maintenance • Efficiently acquired hands-on training for plating dissociated hPSC's, primary mouse embryonic fibroblast (MEF) isolation, MEF feeder layer production, and quality control testing in hPSC cultures -
Graduate Student ResearcherCalifornia State University Channel Islands Feb 2012 - Mar 2012Camarillo, Ca, Us• Optimized the growth conditions of mouse Mesenchymal stem cells (mMSCs) in 3D fibrin scaffold constructed of varying concentrations of fibrinogen and thrombin• Employed live/dead assay and fluorescence microscopy to check the cell proliferation rate of mMSCs -
Graduate Student ResearcherCalifornia State University Channel Islands Jan 2012 - Jan 2012Camarillo, Ca, Us• Checked the effect of over-expression of cyclin-D1 gene on cell proliferation of Chinese Hamster Ovary (CHO) cells.• CHO cells were transfected with cyclin-D1 gene using Lipofectamine-2000 and cell proliferation was checked by using MTS assay
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Research TraineeDisha Life Sciences Dec 2010 - Jun 2011InBlossoming since 2008, Disha Life Sciences Pvt. Ltd. is Renowned name in Gujarat offering at par excellence in academics and nourishing youth for tomorrow's challenge. It is a platform where a student grows to scholar and a scholor grows to a veteran. Disha Life Sciences is equipped with state of art laboratories for Biotechnology, Molecular Biology, Microbiology, Fermentation and Plant Tissue Culture.• Performed molecular characterization of E.coli using various biochemical tests, Gas Chromatography and randomly amplified polymorphic DNA approach• Laboriously practiced basic molecular biology techniques such as polymerase chain reaction, DNA cloning and gel electrophoresis • Proficiently utilized basic microbiology techniques such as inoculating agar plates, growing bacteria on selective media, isolating an organism from the environment, enumerating bacteria using serial dilution, plate counts and spectrophotometer -
Research AssistantPadmashree Dr D. Y. Patil Vidyapeeth Jan 2010 - Jul 2010Pimpri, Pune, Maharashtra, In• Investigated the efficacy of aqueous Aloe vera extract on serum profile and histological examination of pancreas in alloxan-induced diabetic rats. • Determined a novel method for treatment of diabetes in rats followed by statistical data analysis of glucose, protein and lipid levels in rats treated from diabetes.• Anesthetized and isolated pancreas from rats, and performed histological examination to check for the efficiency of herbal treatment in diabetic rats.
Jay Sharma Skills
Jay Sharma Education Details
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California State University Channel IslandsBiotechnology & Bioinformatics -
University At BuffaloBiological Sciences
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Dr. D. Y. Patil VidyapeethBiotechnology
Frequently Asked Questions about Jay Sharma
What company does Jay Sharma work for?
Jay Sharma works for University Of California, San Diego - School Of Medicine
What is Jay Sharma's role at the current company?
Jay Sharma's current role is Staff Research Associate III.
What is Jay Sharma's email address?
Jay Sharma's email address is ja****@****usc.edu
What schools did Jay Sharma attend?
Jay Sharma attended California State University Channel Islands, University At Buffalo, Dr. D. Y. Patil Vidyapeeth.
What are some of Jay Sharma's interests?
Jay Sharma has interest in Children, Environment, Education, Science And Technology, Health.
What skills is Jay Sharma known for?
Jay Sharma has skills like Pcr, Sds Page, Gel Electrophoresis, Molecular Biology, Cell Culture, Biotechnology, Western Blotting, Dna Extraction, Tissue Culture, Confocal Microscopy, Flow Cytometry, Nucleic Acid Extraction.
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