Jean-Christophe Yorke Email & Phone Number

Lyon, Fr

150 Cours Thomas Lyon/ France

Temporary position Duties and responsibilities :- Implementation of the untargeted metabolomics workflow for blood specimens from epidemiological studies. I have optimized the pre-existing workflow for untargeted metabolomics, by including automation of sample preparation and schemes for analysis of large-batches of samples. Robotic extraction of metabolites from blood samples using Perkin Elmer Janus liquid handling system. I have developed a range of robotic methods to perform aliquoting step as well as the extraction steps using a varitety of labwares including eppendorfs, 96-well plates, Agilent Captiva plates and glass vials. Around 3000 blood samples from a range of studies within exposomics and nutritech proejcts were prepared using these automated methods.Operation of Agilent UPLC-QTOF 6550 instrument :- It included routine preparation of the instrument by cleaning ion-source, tuning, calibrating, changing solvents and system suitability tests. Around 2000 samples were analyzed using a standarized LC-MS method. Raw data were processed by me using Agilent softwares Qual, DA Reprocessor and mass profiler professional for quality control checks and generation of peak-lists for each file. I am responsible for maintaining records for operation of the instrument, consumables and other biospecimens storage and handling. Construction of MSMS library for 635 pure standards: I took the initiative to developed a library of MSMS spectra using the standarized LC-MS method on the 6550 QTOF instrument. I acquired the pure standards on 96 well plates from IROA technologies, USA. A chart for analysis was prepared and data acquisition for these standard has been completed for reversed phase column. Participation in scientific symposium: Réseau Français de Métabolomique et Fluxomique -RFMF

Helsinki

Temporary positionDuties and responsibilities :- Installation of new laboratory to perform targeted metabolomics of biological samplesSetting-up of material and methods used to perform quantitative analysis of metabolites in bio fluid samples by using an automated sample preparation system (Hamilton Microlab STAR Line) followed by LC/MS/MS (Waters Xevo TQ-S triple quad).Development and validation of robotic methods for sample preparation and data acquisition for measuring 96 polar compounds in blood.These method allow to cover multi-classes multi-concentration metabolites.Sample preparation takes 2 hours while separation process takes 24 hours for a batch of 96 samples. Sample process for a batch of 96 samples takes 2 days. This method is made available as analytical service by FIMM.Training of 2 junior staffs on targeted metabolomics and overlooking the implementation of the methods by the junior staff. Participation of meetings of Proteomics and Metablomics consortium of the Biocentre Finland and in the eetabomeeting 2011,Helsinki/FI,September 25-28 ,

Helsinki, Finland

Temporary positionDuties and responsibilities :- Development of generic methods by UPLC/Qtof Premier Waters to screen veterinary drugs in food stuffs. Development and validation of methods for milk and meat matrices to replace microbiological or biochemical methods in used for screening veterinary drugs.This method is in used at EVIRA for the screening of anthelminthic drugs. Participated in the meeting for the European experts analysts for veterinary drugs organized in Berlin and to a proficiency test for analyzing Anthelminthics in meat, organized by European union reference laboratory of Berlin.

Caverac/ France- Helsinki/Finland

Marketing , sale and education in the wine business.Promotion and sale of wine makers in Estonian and Finnish MarketParticipation in Viiniexpo 2004 (national Finnish wine fair)Organisation of wine tasting courses for corporate as public

Vergeze, France

Duties and responsibilities :- Overlooking the activities of three sub-ordinate technical staffs for contract research studies perform for pharmaceutics industry. Performed bioanalytical studies following the FDA Guidance for Industry-Bioanalytical Methods-Validation. These analyses were performed with GCMS and LCMSMS (triple quadrupole MRM technology).Planification and follow -up of the studies, validation of the results, proper record of all the data in the GLP study.Three studies for sponsors have been achieved.

Fougeres-France

Temporary positionDuties and responsibilties: Harmonisation of analytical methods for seeking antimicrobials in food stuffs for the European union reference laboratory. These analytical methods are used by the national reference laboratories in Europe to confirm the results found during the screening test.The analytical technic used was Liquid chromatography with fluorescence detection.This position included technical skills as well as management skills and cover all the steps found in the validation of analytical method. I personally cover the full validation of quinolones analysis and transfer this methods to European laboratories.The work included - Method developmentMethod validationMethod Transfer to the European national reference laboratories:Organisation of workshops on these methods Organisation of proficiency test:Production of the reference materialShipment of the test materialAssessment and reporting of lab resultsReporting of the activity to the European national reference laboratoriesParticipation in scientific symposis and preparing publicationI joined the European community reference laboratory at the early stage and participated to the services offer for the European national reference laboratories.

Laboratoire D'Etude Et De Recherche En Environnement Et Sante

Temporary positionDuties and responsibilities :- Technical head of the chromatography unit at the laboratory. Plan and follow-up of analyses of organic micro-pollutants in water, air and soil samples. Unit included 4 technicians analyzing around 8000 samples per year.The chromatographic equipment included : 2 xGC MS, 4 GC (ECD,NPD, FID), 1 SFE, 1 automate for samples preparation, 1 LC/Fluo, 1 LC/UV. My work was to develop methods for - Increasing of laboratory productivity and the safety with automatisation of sample preparation. The liquid/liquid extraction were replaced by solid phase extraction .Sample preparation with SFERoutine analysis with GC/MS, GCDevelopment LC Fluo analysisAnalytical support provide to Master studentsParticipation of laboratory equipment purchaseManagement of the chromatographic equipment

Service Hospitalier Fredericjoliot,Orsay/France

Permanant positionDuties and responsabilities :- Labelling of medicinal drug with short half time time radio isotope 11C, 18F, 15O used for studying in vivo organs like brain and heart with Positron Emission Tomography.Routine analysis, included short organic synthesis and separation of the labelled drugs with HPLC column detection UV and gamma .Work was performed by remote control in shield hood.Development of a new synthesisAutomatisation for the synthesis of the 18F Fluoro Deoxy Glucose(18 FDG).I developped the first automate for producing the 18 FDG. Nowadays the production of 18 FDG is achieved worldwide using commercial automate. 18 FDG is now the standard radiotracer used for PET neuroimaging and cancer patient management.

D.E.S.S Instrumentation Et Méthodes Physico-Chimiques D’Analyse,Faculté D’Orsay, Analytical Chemistry

- Analysis of organic compounds using separation technics and detection with spectrometric methods - Practical training on NMR, MS, UV-VIS.

Diplome D'Etudes Superieures Techniques, Organic Chemistry, Master

Main study; Synthesis of organic compounds Analytical chemistry applied to the synthesis Chemical engineering

Diplome Universitaire De Technologie, Chemistry

Fondamental courses on chemistry