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I am a lab based scientist with a strong background in cytokine and cellular immunology. My day-to-day work primarily involves the design and evaluation of robust cell-based bioassays that are used to assess the potency of biologics at various stages of clinical development. In this role, I regularly engage in cross-functional CMC analytical working groups to support sample testing, assay transfer and regulatory filings.
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Sr Scientist | Biologics Analytical R And D | Cell Potency AssaysMerckRahway, Nj, Us -
Sr Scientist | Biologics Analytical R&D | Cell Potency AssaysMerck Apr 2020 - PresentKenilworth, New Jersey, United StatesI am currently part of a cross-functional team operating in the CMC space to develop phase-appropriate bioassays to assess the potency of biologics. This work is underpinned by a strong understanding of the "mechanism of action" for each drug and its manufacturing process. This informs the identification of critical quality attributes (CQA) that affect potency, and helps to control process. -
Sr. Scientist | Preclinical Development | Cell EngineeringMerck Feb 2020 - Apr 2020Kenilworth, New Jersey -
Postdoctoral FellowFrederick National Lab For Cancer Research (Operated By Leidos Biomedical Research Inc.) Sep 2016 - Feb 2020Frederick, MdProject 1) Utilizing an adoptive cell therapy approach in non human primates, our lab is studying factors that influence an effective antiviral T cell response during AIDS-virus infection. Through the engineered expression of cloned T cell receptors (TCR) and homing receptors, we are able to confer target specificity and directed localization to infused autologous T cells. Using this strategy, we aim to eliminate viral reservoirs in the lymph nodes of SIV infected rhesus macaques. More broadly, through this immunotherapy process, we are gaining translatable insights into the various qualitative factors that determine the efficacy and persistence of adoptively transferred cells. Project 2) In collaboration with the Viral Oncology Section at the LBR AIDS and Cancer Virus Program, we are also studying the highly varied human T cell responses to Kaposi's sarcoma-associated herpesvirus. Using an in vitro experimental approach, we are cloning virus-specific TCRs that are isolated from sero-positive individuals, and studying TCR clonotype avidity using functional immunoassays. From these studies, we aim to identify key T cell factors that determine an effective T cell response, and potential correlates of immune protection.
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Ph.D. ImmunologyUniversity Of Toronto Sep 2006 - Apr 2015University Health Network*Utilized a mouse model of viral myocarditis to identify a novel synergistic effect between the type II diabetes drug (metformin) and IFN-beta to enhance an antiviral response.*Studied the influence of type I IFNs on protein synthesis and glucose metabolism during an antiviral immune response*Contributed expertise in murine infection models towards local and international collaborations -
Research Assistant | Trait And Technology DevelopmentDupont Pioneer May 2005 - Aug 2006Georgetown, On*Assisted in the design and analysis of PCR-based mapping of a Canola breeding line *Contributed to the development of transgenic glyphosate-tolerant Canola
Daniel Burke Skills
Daniel Burke Education Details
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Immunology -
Microbiology
Frequently Asked Questions about Daniel Burke
What company does Daniel Burke work for?
Daniel Burke works for Merck
What is Daniel Burke's role at the current company?
Daniel Burke's current role is Sr Scientist | Biologics Analytical R and D | Cell Potency Assays.
What is Daniel Burke's email address?
Daniel Burke's email address is da****@****onto.ca
What is Daniel Burke's direct phone number?
Daniel Burke's direct phone number is +136173*****
What schools did Daniel Burke attend?
Daniel Burke attended University Of Toronto, University Of Guelph.
What are some of Daniel Burke's interests?
Daniel Burke has interest in Kayaking, Gardening, Aquaponics, Education, Immunology, Welding, Metal Casting, Hiking, Science And Technology, Sculpture.
What skills is Daniel Burke known for?
Daniel Burke has skills like Immunology, Animal Models, Cell Culture, Western Blotting, Virus Culture, Molecular Biology, Qpcr, Pcr, Infectious Diseases, Rt Pcr, Real Time Pcr, Rna Isolation.
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Daniel Burke
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