Jefferson Puerta Email and Phone Number

• Translate User needs into system and requirements (hardware, software, consumable, biological, pharmaceutical testing).• lead instrumentation development projects to improve system functionality and robustness.• Work across multiple disciplines within organization (engineering, biology, product management, field support, quality, manufacturing) to nurture overall system design of product.• Contribute to data-driven decision making by designing experiments and collecting, analyzing, and presenting data results.• Lead system level troubleshooting of products (hardware, software, consumables, protocols).• Contribute to development of design concepts and solutions to complex technical problems.

• Design and test new prototypes for engineering functionality of instrumentation.• Design and test new engineering prototypes for biological function.• Assisted in design of new instrumentation.• Assisted in small-scale production of engineering prototypes.• Validated instrumentation and consumables for biological use.• Trained new users on how to use instrumentation and Organ-Chips.

• Cultured Caco 2 BBE cells in microfluidic Organ-Chips to develop models for the small intestine.• Planned and performed experiments on Intestine-on-Chip model• Analyzed data gathered from experiments including cell imaging and cell viability and permeability assays. • Developed protocols for the Intestine-on-chip model (Chip Imaging, ECM Coating, Assay Development and measuring Transepithelial Electrical Resistance (TEER).• Performed assays (cell viability and permeability) on samples from both transwells and Organ-Chips.

• Fabricated microfluidic devices for the Organs-on-Chips research team out of polydimethylsiloxane (PDMS).• Optimized and documented protocols for fabricating microfluidic devices.• Optimized protocol for fabricating thin porous PDMS membranes used in Organ-on-Chips• Designed and built a device that automates process of fabricating thin porous PDMS membranes.• Trained new users on developed microfabrication protocols.• Wrote standard operating procedures (SOP) on new and updated protocols• Cultured Caco-2 cells in microfluidic devices for the Gut-on-a-Chip model.